Anti Versican/CSPG2 (5C12) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Versican/CSPG2 (5C12) Antibody

Catalog No.: CAC-PRPG-VS-M01
Size: 2ML
Price: ¥50000
$667
antigen/source: Versican/CSPG2
host: Mouse
Label: Unlabeled
Purity: Supernatant
Application: Western Blot
IHC frozen section
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Immunogen: Human
Isotype: IgM
Clone: 5C12
Reacts with: Human
Purpose: Western blotting: 1/20 - 1/60
Immunohistochemistry: 1/25 - 1/75 (PFA-frozen sections)
ELISA: 1/50 - 1/150
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Other:

 Background 
Versican, originally also known as PG-M, and encoded by the VCAN/CSPG2 gene, is a large extracellular matrix chondroitin sulfate proteoglycan ubiquitously expressed in interstitial matrices of the human body including that of brain. It was first isolated from the bovine aorta by Dick Heinegard's and Anders Malmstrom's groups (1982) and shortly after isolated from the chick embryonic limbs by Koji Kimata's group (1986). Cloning of the human VCAN/CSPG2 gene was accomplished in 1989by Zimmermann and Ruoslahti, who also cognated the name versicanin recognition of its versatile modular structure.
 Cross reactivity 
Human, Bovine
Other species have not been tested.



 IMAGE1



(A) Immunoblotting of intact versican (mixture of V1 and V2 isoforms) prior to (Intact) and after combined chondroitinase ABC (Chase ABC) and endo- -galactosidase-digestion (Digested) form. The proteoglycan was resolved by SDS-PAGE under reducing conditions on 3-8% linear gradient gels (MW, HiMark Unstained Protein Standard). Banding pattern depends upon the isoforms and is often complex. In the intact forms, i.e. without removal of GAGs, isoforms V0, V1 and V2 do not enter conventional polyacrylamide gels and therefore alternative gel types are strongly recommended. Following chondroitinase-digestion and extensive enzymatic deglycosylation, most isoforms still show complex, smeared banding patterns. (B) Immunohistochemistry on human normal urinary bladder. (C) Immunostaining of versican in the matrix deposited in vitro of human microvascular endothelial cells after TNF stimulation. (D) Immunostaining of versican lining the wall of a larger vein in human kidney (paraffin section).

 IMAGE2





Reference:

•  Mazzucato M. et al., FASEB J. 16 1903-1916 (2002)
•  Cattaruzza S. et al., J.Biol.Chem. 277 47626-47635 (2002)
•  Cattaruzza S. et al., Matrix Biol. 24 400-417 (2005)
 
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