Anti His6 Tag - Cosmo Bio Co.,Ltd.



Anti His6 Tag Antibody

Catalog No.: BAM-60-051-EX
Size: 100UL
Price: ¥22000
antigen/source: His6 Tag
host: Rabbit
Purity: IgG fraction
Application: Western Blot
Enzyme Linked Immunosorbent Assay
catalog info: Catalog 2012-p50
Storage: -20C
Purpose: Tag protein antibodies.


 Background Epitope tagging has become a powerful tool for detection and purification of expressed proteins. His6-epitope tag is especially useful because the tagged recombinant proteins can be easily purified by affinity chromatography with metalion (e.g.Ni2+, Co2+ etc.)) conjugated resins. Due to their small size, His epitope tag does not affect the tagged protein's biochemical properties in most cases.
Anti-His epitope tag antibody is useful for identification of a recombinant protein tagged with 6X His at N-terminal, C-terminal and internal of the proteins.


Fig. Detection of His-tagged proteins (FLAG-His6-EmGFP, FLAG-His6-EmGFP-PRMT6) in crude extract of 293T cells expressing the recombinant proteins with anti-EGFP antibody (left panel) and anti-His6 antibody (right panel). The antibodies were used at x 1000 fold dilution.


•  Simpson RJ, Adams PD and Golemis EA. ColdSpring Harbor Laboratory Press (2009) New York