Dityrosine (DT) ELISA kit - Cosmo Bio Co.,Ltd.

Antibodies

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Dityrosine (DT) ELISA kit

Catalog No.: NNS-KDT-010E-EX
Size: 96WELL
Price: ¥80000
$1067
antigen/source: Dityrosine
catalog info: Catalog 2012-p111
Storage: 4C
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Immunogen: Human
Reacts with: Human
Measurement Range: 0.05 - 12 umol/L
Tag: Urine
Purpose: Dityrosine is one of the specific biomarkers for protein oxidation. This DT ELISA kit is designed for quantitative measurement of DT especially in urine samples.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. DT Microtiter Plate Precoated with DT (8x 12 wells, split type) 1 plate
2. Primary Antibody Anti DT monoclonal antibody (ready to use) 1 vial (7 mL )
3. Secondary Antibody HRPconjugated antimouse antibody 1 vial
4. Secondary Antibody Solution Phosphate Buffered Saline 1 vial (12 mL )
5. TMB Substrate Chromogen (ready to use) 1 vial (12 mL )
6. Stop Solution 1.96% Sulfuric acid (ready to use) 1 vial (12 mL)
7. Washing Solution (x5) Concentrated wash buffer 3 vials (25 mL x 3)
8. DT Standards DT standards (ready to use) 1 vial each (0.5 mL )
9. Plate seal 2 sheets

Other:

 Supplementary 
Competitive
 Applicable sample 
Urine
[Other]
Tyrosine is one of the major targets of protein oxidation, and until today various tyrosine derivatives such as nitrotyrosine, dityrosine and halogenated tyrosine depending on the type of free radicals. DT is a tyrosine dimer derived from tyrosyl radicals which is formed by reactive oxygen species (ROS), metal-catalyzed oxidation, ultraviolet irradiation, and peroxidases. DT have been found in atherosclerotic lesions, and lipofuscin of pyramidal neurons of aged human brains. Dityrosine is one of the specific biomarkers for protein oxidation.
Recently, dityrosine is reported to exist also in urine samples. It is expected that DT may be a novel protein oxdation marker,which is non-invasively detectable.



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A standard curve example

Reference:

•  Kato Y, Wu X, Naito M, Nomura H, Kitamoto N, Osawa T. Biochem Biophys Res Commun. 275(1), p11-15 (2000).
 
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