Anti TRH Toxin - Cosmo Bio Co.,Ltd.

Antibodies

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Anti TRH Toxin Antibody

Catalog No.: BAM-64-015EX
Size: 100UG
Price: ¥34500
$460
antigen/source: TRH Toxin
host: Rabbit
Description: [Species]Vibrio parahaemolyticus
Label: Unlabeled
Purity: Immunogloblin fraction
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Immunoprecipitation
Storage: -20C
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Immunogen: Vibrio

Other:

 Background 
Many Vibrio parahemolyticus isolated as a cause of food poisoning, produce toxin called hemolysin, and this is the
main cause of illness. The hemolysin of V. parahemolyticus mainly interacts with an intestinal tract or the heart,
produces diarrhea by enterotoxicity, and also there are severe cases of making a patient die by cardiotoxicity. Two
kinds, heat-resistant hemolysin (TDH, thermostable direct hemolysin) and heat-resistant toxin related hemolysin
(TRH, TDH-related hemolysin), areknown as hemolysins of V. parahemolyticus. Among these, TDH is known for
many years and has been studied more extensively. In order to distinguish whether it is the V. parahemolyticus that
produces TDH, samples are grown on the Agatsuma medium (blood agar which is added with mannitol), and
judged by whether a hemolysis is observed or not. This hemolysis was one of the examining methods, which is
called Kanagawa phenomenon to judges whether it is pathogenic V. parahemolytica.However, itbecame clear that
the food poisoning by the V. parahemolyticus of Kanagawa-phenomenon negativity was discovered, and this
organism did not produce TDH, but it produced TRH. Moreover, since the Kanagawa phenomenon sensitivity is
notso high, theimmunological technique employing antibody against toxin is used together for the judgment of
pathogenicity of V. parahemolyticus.
TRH is the heat labile toxin protein of molecular weight 21.1 kDa (189 aa). Homology of TDH (21.3kDa,189 aa)
with TRH is about 60% (reference 1 and 2), and shows partial antigenic similarities. Susceptibility of the blood
cells of various animals to TRH differs greatly, and TRH shows more than 100 times in rabbit skin capillary
permeabilityactivity than TDH.
 Application 
1) Western blotting (2,000~10,000 time dilution) (Fig. 1)
2) Immunoprecipitation (assay dependent) (Fig. 2)
3) ELISA (assay dependent)



 IMAGE1



Fig. 1. Detection of TRH toxin in the culure medium of V. parahaemolyticus (TRH+) by western blotting with anti-TRH antibody. 1. Ten-times condensed sample were analyzed by SDS-PAGE and stained with CBB. 2. The same sample was analyzed by western blotting. The antibody at 1/2,000 dilution was used. TRH toxin band is indicated by an arrow

 IMAGE2



Fig. 2. Immunoprecipitation of TRH toxin from Vibrio parahaemolyticus (TRH+) culture medium with anti-TRH antibody. 1. TRH was precipitated with anti-TRH antibody from the same sample as in Fig.1, analysed by SDS-PAGE and stained with CBB. 2. TRH was precipitated with anti-TRH antibody from the same sample and TRH was detected by WB with anti-TRH antibody. Arrow indicates TRH band. H and L indicate heavy and light chain of IgG, respectively.

 IMAGE3



Fig.3. Anti-TRH antibody cross-reacts with TDH toxin in western blot. 1. Partially purified TDH toxin 2. Condensed culture medim of V. haemolyticus. Antibody was used at 1/2,000 dilution for WB.

Reference:

•  Honda T. et al., Infect. Immun. 56 :961-965(1988). PubMed ID 3126151 Free access
•  Nishibuchi M et al., Infect Immun 57:2691-7(1989), PubMed ID 2759706 Free access
 
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