Fig.1.Detection of VT1 by western blotting with anti-VT1
1. SDS-PAGE of culture medium of VTEC, stained with CBB
2. SDS-PAGE of crude extracts of VTEC cells, stained with
3. Western blotting of culture medium of VTEC
4. Western blotting of crude extracts of VTEC cells.
Ani-VT1 antibody was used at 1/2,000 dilution
Fig. 2. Immunoprecipitation of VT1 from culture
medium of VTEC with anti-VT1 antibody.
Arrows shows subunit A and subunit B of VT1.
Heavy chain and Light chain indicate those of IgG.