Anti DNA polymerase eta - Cosmo Bio Co.,Ltd.



Anti DNA polymerase eta Antibody

Catalog No.: BAM-70-070EX
Size: 20UG
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: DNA polymerase eta
host: Mouse
Label: Unlabeled
Purity: Purified
Application: Western Blot
Immuno Fluorescence
Storage: -20C
Immunogen: Human
Isotype: IgG1
Clone: 5H10
Reacts with: Human


 Features :
DNA polymerase eta ( 713 aa, 78 kDa) is specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high fidelity DNApolymerases cannot proceed and DNA synthesis stalls. Plays an importantrole in the repair of UV-induced pyrimidine dimers. Depending on the context, it inserts the correct base, but causes frequent base transitions and transversions. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity. Targets POLI (Pol iota) to replication foci.
 Application :
Western blot. Not tested for other application
Human recombinant full-size Pol eta tagged with His6.


Fig.1. Identification of Pol eta in whole cell extract of HeLa cells by western blot with anti-Pol eta antibody (5H10). Lane 1; Recombinant full-size Pol eta with His6 tag at C-teminus. Positive control. Lnae 2; Whole cell extract of HeLa cells. ~40 ug protein applied. Positive control Lane 3; Whole cell extract of XP2SA (XP-V) cells. ~40 ug protein applied. Negative control PoL eta is detected at ~80 kDa position. 8% gel was used. Data by courtesy of Prof. F. Hanaoka and Prof. C. Masutani at Osaka University.


Fig.2. Immunofluorescence staining of DNA polymerase eta in HeLa cells with anti Pol eta antibody. HeLa cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. anti-Pol eta antibody was used at 1/1,000 dilution.