Anti Nup98 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Nup98 Antibody

Catalog No.: BAM-70-345EX
Size: 100UG
Price: ¥34500
$460
antigen/source: Nup98
host: Mouse
Description: [Species]Tetrahymena thermophila
Label: Unlabeled
Application: Western Blot
Immuno Fluorescence
Immunocytochemistry (cell)
Storage: -20C
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Immunogen: Tetrahymena
Isotype: IgG1
Clone: 13C2
Reacts with: Human
Tetrahymena

Other:

 Background 
Nucleoporin 98 (Nup98) is a component of nuclear pore complex (NPC), which is a large protein
assembly embedded in the nuclear envelope and highly conserved in eukaryotes. It is localized on
both nuclear and cytoplasmic side of NPC.
This protein contains glycine-leucine-phenylalanine-glycine (GLFG) amino acid repeats and
plays a critical role in nuclear trafficking. Nup98 also plays a specific role in the RNA export. In
addition, Nup98 plays roles in several important biological events such as gene expression, mitotic
checkpoint, and pathogenesis
Nup98 gene is fused to a variety of partner genes in human myeloid and T-cell malignancies via
chromosomal translocation.
In ciliates, a unicellular organism havingtwo functionally distinct nuclei, GLFG-Nup98 is present
in one of the nuclei and a distinct Nup98 ortholog is present in the other nucleus, and these
different Nup98s participate in a nucleus-selective transport mechanism.
 Application 
1) Most suitable for Western blotting, (Human, S. cerevisiae, S. pombe, Tetrahymena. Not tested in
other species)
2) Immunofluorescence staining / Immunocytochemistry for S. cerevisiae, S. pombe and
T.etrahymena. Not suitable for IF in human




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Fig.1 Immunofluorescene staining of Nup98 in HeLa cells using 13C2 , 21A10 , or 2H10 monoclonal antibodies.

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Fig.2. Western blot analysis of Nup98 in HeLa cells with anti-Nup98 antibodies

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Fig.3 Immunofluorescene staining of Nup98 in Tetrahymena themophila cells using 13C2 , 21A10 , 2H10 or 414 monoclonal antibodies.

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Fig.4 Detection of MacNup98A of Tetrahymena by Western blotting with monoclonal antibodies 13C2 and 21A10.

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Fig.5 Immunofluorescene staining of Nup98 in S. pombe cells with 13C2 , 21A10, or 2H10 monoclonal antibodies.

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Fig.6 Detection of Nup98 in S. pombe cell extracts by Western blotting with monoclonal antibodies 13C2, 21A10 and 2H10.

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Fig.7 Monoclonal antibodies 13C2 and 21A10 crossreact with multiple nucleoporins of S. cerevisiae. (A) IF staining of S. cereviciae cells using 13C2, 21A10, or 2H10 mAbs (B) WB analysis of S. cereviciae cell extract using 13C2 , 21A10, or 2H10 mAbs

Reference:

◊  Iwamoto M. et al., (2013) Monoclon Antib Immunodiagn Immunother. 32: 81-90 PubMed ID: 23607342 WB, IF
•  Iwamoto M. et al., (2010) Genes Cells. 15: 661-9. PubMed ID: 20545767 Free article. Review
 
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