Total GLP-1-HS ELISA - Cosmo Bio Co.,Ltd.

Antibodies

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Total GLP-1-HS ELISA

Catalog No.: YII-YK161-EX
Size: 1KIT
Price: ¥70000
$934
antigen/source: Total GLP-1-HS
Application: Enzyme Linked Immunosorbent Assay
Storage: 4C
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Reacts with: Rat
Human
Measurement Range: 1.24 ] 300pM
Purpose: For quantitative determination of total GLP-1 in rat and human plasma sample. The kit is characterized by sensitive quantification and high specificity.
component: Antibody: X
Plate: X
Coating: X
Control: -
Standard: X
Labeling: X
Substrate: X
Others: X
1. Antibody coated plate : 1 plate (96 wells)
2. Standard : 1 vial (300 fmol)
3. Biotin labeled antibody solution : 1 bottle (12 mL )
4. SA-HRP solution : 1 bottle (12 mL )
5. Enzyme substrate solution : 1 bottle (12 mL )
6. Stopping solution : 1 bottle (12 mL )
7. Buffer solution : 1 bottle (20 mL )
8. Washing solution (concentrated) : 1 bottle (50 mL )
9. Adhesive foil : 4 pieces

Other:

 Supplementary 
Sandwich
 Applicable sample 
plasma and culture medium supernatant
[Other]
This ELISA kit has high specificity to GLP-1(1-36) amide, GLP-1(7-36) amide and GLP-1(9-36) amide and shows low crossreactivity to GLP-1(1-37) and GLP-1(7-37), and shows no crossreactivity to rat and human GLP-2, rat Glicentin, human Glucagon, rat mouse and human GIP (1-42), rat and human GIP (3-42).
 Cross reactivity 
GLP-1 (7-36) amide : 100%
GLP-1 (9-36) amide : 100%
GLP-1 (1-36) amide : 100%
GLP-1 (1-37) : 9.1%
GLP-1 (7-37) : 9.4%
Rat GLP-2 : <0.1%
Human GLP-2 : <0.1%
Rat Glicentin : <0.1%
Human Glucagon : <0.1%
Rat GIP (1-42) : <0.1%
Mouse GIP (1-42) : <0.1%
Human GIP (1-42) : <0.1%
Rat GIP (3-42) : <0.1%
Human GIP (3-42) : <0.1%


Reference:

•  Heinrich, G. J. Biol. Chem., 14082-14087 (1984)
•  Orskov, C. Diabetes., 535-539 (1994)
•  Yanaihara N. Hum. Cell., 1-8 (1990)
•  Asarian, L. Physiol. Behav., 367-372 (1998)
•  Mojsov, S. J. Biol. Chem., 8001-8008 (1990)
•  Vella,A. Diabetes., 611-617 (2000)
•  Nauck, MA. Diabet. Med., 937-945 (1998)
•  Nauck, MA. Regul. Pept., 89-97 (2004)
•  Naslund, E. Br. J. Nutr., 439-446 (2004)
•  Holst, JJ. Diabetes Metab. Res. Rev., 430-441 (2002)
 
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