Anti DNA polymerase 1 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti DNA polymerase 1 Antibody

Catalog No.: BAM-61-012EX
Size: 100UL
Price: ¥34500
$460
antigen/source: DNA polymerase 1
host: Rabbit
Label: Unlabeled
Purity: Serum
Application: Western Blot
Immunoprecipitation
Storage: -20C
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Immunogen: Escherichia coli
Reacts with: Escherichia coli
Shape: Liquid

Other:

 Application 
1. Western blot. (1/1,000~ 1/5,000 dilution)
2. Immunoprecipitation (assay dependent)
Not tested for other applications
 Cross reactivity 
E. coli DNA polymerase 1. Not tested in other species



 IMAGE1



Fig.1 Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using anti-DNA polymerase 1 antibody. 1; Purified E. coli DNA polymerase 1 (10 ng) 2; Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1/2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.

 IMAGE2



Fig.2 Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with anti-DNA polymerase 1 antibody 1. Purified DNA polymerase 1 ( 20 ng), WB 2. Purified DNA polymerase 1 (10 ng), WB 3. Crude lysate of E. coli strain AB1157 (10 µg), WB 4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP for IP (Abcam 131368) was used.
 
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