Anti Pertussis Toxin - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Pertussis Toxin Antibody

Catalog No.: BAM-64-030EX
Size: 100UL
Price: ¥34500
$460
antigen/source: Pertussis Toxin
host: Rabbit
Label: Unlabeled
Purity: Serum
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Dot Blot
Immunoprecipitation
Neutralising
Storage: -20C
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Other:

 Background 
Perrtussis toxin (PT) is a protein-based AB5-type exotoxin produced by Bordeterra pertussis. PT catalyzes the
ADP-ribosylation of the subunits of the heterotrimeric guanine nucleotide regulatory proteins Gi, Go, and Gt and
prevents intracellular signal transduction involving the G proteins. PT consists of one moplecule of each S1 (26
kDa), S2 (22 kDa), S3 (22 kDa), S5 (12 kDa) and two molecule of S4 (12 kDa). This product was highly purified
(>90% pure) from Bordetellapertussis strain Tohama by the method of Skelton & Wong1). Cytotoxicity of the PT was
confirmed by morphological alteration of CHO cells after treatment with 0.1 ng/ml of PT (see the Figure below).
 Application 
1. Western blotting (1/2,000~1/10,000 dilution)
2. ELISA (1/10,000~1/20,000 dilution)

3. Dot blotting (1/2,000~1/10,000 dilution)
4. Immunoprecipitation (1/200~1/500 dilution)
5. Neutralising (Assay dependent)
Other applications have not been tested.
[Immunogen]
Immunization was Initiated with toxoid and boosted with native toxin (BioAcademia 01-503)



 IMAGE1



Fig.1. Detection of perussis toxin in culture medium of Bordetera pertussis strain Tohama by Western blotting using anti-perussis toxin antibody. 1. Culture medium of Bordetera pertussis. SDS-PAGE, silver-stained 2. Purified pertussis toxin (200 ng). SDS-PAGE, silver-stained 3. Western blot of culture medium of Bordetera pertussis as in 1. 4. Western blot of purified pertussis toxin (10 ng) The toxin consists of five subunits as indicated by S1 to S5.

 IMAGE2



Fig.2. Titration of antibody reactivity of anti-Pertussis antiserum by direct ELISA Plate was coated with 100 µg of pertussis toxin per well and 100 µl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody.

 IMAGE3



Fig.3. Titration of pertussis toxin by direct ELISA using anti-pertussis toxin antiserum ELISA plate is coated with indicated amounts of pertussis toxin per well. Antiserum was used at 1/12,500 dilution. ELISA was performed as in Fig.2. Dynamic range was 100 pg to 10 ng under these conditions.

Reference:

•  Alouf JE & Popoff MR (Ed.), Toxins 3rd Ed. Academic Press (2006)
 
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