Anti Influenza A Virus Nucleoprotein - Cosmo Bio Co.,Ltd.

Antibodies

>

Anti Influenza A Virus Nucleoprotein Antibody

Catalog No.: BAM-65-111EX
Size: 50UG
Price: ¥34500
$460
antigen/source: Influenza A Virus Nucleoprotein
host: Mouse Mono
Label: Horseradish Peroxidase
Application: Western Blot
Immunocytochemistry (cell)
Immunohistochemistry
Storage: -20C
@
Clone: C43

Other:

 Background 
Influenza virus is an RNA virus, which causes influenza, and belongs to the family Orthomyxoviridae.
Influenza virus is classified into three different genera, influenzavirus A, B, and C. They all have similar structures
and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the
virion is made of a viral envelope containing two major glycoproteins, hemagglutinin (HA) and neuraminidase (NA).
Influenzavirus A is further classified into subtypes based on the surface glycoproteins, HA and NA. Currently, there
are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome, which is packaged in
the form of ribonucleoprotein complexes.
Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly
expressed during the course of infection. It is a structural protein, which encapsidates the negative strand viral RNA
and is essential for RNAtranscription, replication and packaging. NP binds the PB1 and PB2 subunits of the viral
RNA polymerase and the matrix protein M1, in addition to its binding to ssRNA. NP is also known to interact with
variety of other macromolecules of both viral and cellular origins, and these interactions have been shown to be
essential for the viral lifecycle.
 Application 
1) Western blotting (300~1,000 fold dilution)
2) Immunocytochemistry (~200 fold dilution)
4) Immunohistochemistry (~200 fold dilution)
5) ELISA (assay dependent)
[Immunogen]
an Influenza A Virus (H2N2) Okada strain



 IMAGE1



Figure. Western blotting of MDCK cells infected with H1N1 (A/PuertoRico/8/34), H5N1 (A/duck/HK/342/78), or H5N2 (A/crow/Kyoto/53/04) using C43 antibody. Samples were collected at 3, 9, 24, and 48 hours post-infection. C43 detected NP after 3 hours post-infection and detected three different types of influenza viruses.

Reference:

◊  Mizuike R. et al., Clin Vaccine Immunol 18: 494-499 PubMed ID: 21228147 (IF/ICC) (2011)
◊  Ueda M. et al., Virus Research 136: 91-97, PubMed ID:18550190 (WB) (2008)
◊  Okuno Y. et al., J Virol 67: 2552-2558 PubMed ID:7682624 (IP) (1993)
 
>