Anti GRWD1 - Cosmo Bio Co.,Ltd.

Antibodies

>  

Anti GRWD1 Antibody

Catalog No.: BAM-70-130EX
Size: 100UG
Price: ¥34500
$460
antigen/source: GRWD1
host: Rabbit
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
Immuno Fluorescence
Storage: -20C
@
Immunogen: Human
Reacts with: Human
Mouse
Rat

Other:

 Background 
Glutamate-rich WD repeat-containing protein 1 (GRWD1) consists of 446 amino acids with
molecular mass of 49.4 kDa. It has been found as a protein which interacts with METTL18 and CDT1 proteins.
It has been implicated in regulationof DNA replication and/or in ribosome biogenesis.
 Application 
1. Western blotting (1/1,000~1/3,000 dilution)
2. Immunoprecipitation (Assay dependent)
3. Immunofluorescence staining / Immunochemistry (1/100~1/1,000 dilution)
[Immunogen]
Purified GST-GRWD1 (human, full-length) expressed in E. coli
 Cross reactivity 
Human, mouse and rat. Other species have not been tested.



 IMAGE1



Fig.1. Identication of GRWD1 proteins in whole cell lysates by western blotting with anti-GRWD1 antibody Whole cell lysates of HeLa cells untreated (-) and treated (+) with DNA damaging agent, adriamycin (Ad), and NIH3T3 cells were analyzed by western blotting with anti-GRWD1 antibody at 1/1,000 dilution. The samples were 10 µg. Second antibody was HRP-conjugated goat anti-rabbit IgG used at 1/5,000 dilution. The revelation of multiple bands indicates post-translational modification such as phosphorylation. The level of GRWD1 in the cell was not affected by DNA-damaging treatment. The identity of an additional band at ~85 kDa position other than the GRWD1 band in NIH-3T3 cell lysate is not known. The GRWD1 proteins were identified at a position higher (~55 kDa) than expected from the molecular mass of GRWD1 indicated from cDNA sequence (49.4 kDa).

 IMAGE2



Fig.2. Immunofluorescence staining of GRWD1 protein in HeLa cells. Hela cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-GRWD1 antibody was used at 1/1,000 dilution. As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/5,000 dilution. As a signal enhancer, Can Get Signal Immunostain B (Toyobo, Osaka) was used according to the protocol of the supplier. Nuclei were stained with DAPI. GRWD1 protein is localized in nuclei.
 
>