Anti CDC6 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti CDC6 Antibody

Catalog No.: BAM-70-133EX
Size: 100UG
Price: ¥34500
$460
antigen/source: CDC6
host: Rabbit
Application: Western Blot
Immuno Fluorescence
Storage: -20C
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Other:

 Background 
CDC6 (Cell Division Control Protein 6 homolog) (human; 560 aa, 62.7 kDa) is involved in the initiation of
DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is
initiated.
Subcellular location: The protein is nuclear in G1 and cytoplasmic in S-phase cells.
 Application 
1. Western blotting (1/1,000~3,000)
2. Immunoprecipitation iAssay dependentj
3. Immunofluorescence staining
[Immunogen]
Recombinant GST-human CDC6 (1-326 amino acids) expressed in E. coli and purified.



 IMAGE1



Fig.1 Identification of CDC6 protein in whole cell lysate of HeLa cells by western blotting using anti-CDC6 antibody. Lane 1; Size marker proteins (kDa) Lane 2; HeLa cell whole lysate (10 µg) Anti-CDC6 antibody was used at 1/1,000 dilution.

 IMAGE2



Fig 2. Immunoprecipitation of CDC6 protein from whole cell lysate of HeLa cells by using anti-CDC6 antibody. CDC6 protein was precipitated from whole cell lysate of HeLa cells with anti-CDC6 antibody beads and probed with anti-CDC6 antibody by western blotting.. 1; Control IP with non-immune IgG 2; Immunoprecipitates with anti-CDC6 antibody 3; Input (whole cell lysate)

 IMAGE3



Immunofluorescence staining of CDC6 protein in HeLa cells with anti-CDC6 antibody, Confocal microscopic analyses of localization of CDC6 proteins in HeLa cells in G1 or early S phase. Cells in mid to late G1phase or hydroxyurea-treated early S phase were fixed with formaldehyde directly or after extraction with Triton X-100 and then immunostained with anti-CDC6 or control non-immune antibody (Alexa;green). The samples were further treated with propidium iodide for DNA staining (PI; red).

Reference:

◊  Fujita M. et al., J Biol Chem. 274:25927-32. WB, IP, IF, (1999)
◊  Fujita M. et al., J Biol Chem. 277:10354-61. WB, IP, IF, (2002)
◊  Tatsumi Y. et al., J Cell Sci. 119:3128-40. WB, Freearticle (2006)
◊  Sugimoto N. et al., J Cell Sci. 5;:1184-91. WB, (2009)
 
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