Anti Brg1 - Cosmo Bio Co.,Ltd.

Antibodies

>

Anti Brg1 Antibody

Catalog No.: BAM-70-230EX
Size: 100UG
Price: ¥34500
$460
antigen/source: Brg1
host: Rabbit
Storage: -20C
@

Other:

 Application 
1) Western blotting (1/1,000 dilution) (Fig. 1)
2) Immunoprecipitation (Assay dependent) (Fig. 2)
3) Immunofluorescence staining (1/1,000 dilution) (Fig. 3)
Not tested for other applications.
 Cross reactivity 
Human and mouse. Not tested in other species



 IMAGE1



Fig.1 Identification of Brg1 in whole cell extracts of human and mouse cells by western blotting using anti-Brg1 antibody. Lane 1; Protein size makers (kDa) Lane 2; HeLa (human) cell extract Lane 3; NIH 3T3 (mouse) cell extract * Star indicates the position of Brg1 protein bands (Predicted molecular mass of Bgr1 is 185 kDa). Anti-Brg1 antibody was used at 1/1,000 dilution

 IMAGE2



Fig.2 Silencing of Brg1 expression by specific siRNA for Brg1. HeLa cells were transfected with the indicated siRNAs. Forty-eight hours after transfection, whole cell lysates were analyzed by western blotting. The signal intensities of the bands were quantified and shown below the blot, with control siRNA-treated cells as 100 %.

 IMAGE3



Fig 3. Immunoprecipitation of Brg1 protein from nuclear extracts of HeLa cells by using anti-Brg1 antibody. Brg1 was precipitated with anti-Brg1 antibody and proteinG-conjugated agarose beads and probed with anti-Brg1 antibody by western blotting.. Lane1; Input nuclear extracts Lane 2; Control IP with non-immune IgG Lane 3; Immunoprecipitation with anti-Brg1 antibody

 IMAGE4



Fig.4. Immunofluorescence staining of Brg1 protein in HeLa cells. Hela cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-Brg1 antibody was used at 1/1,000 dilution. As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/1,000 dilution. Nuclei were stained with DAPI. Brg1 protein is localized in nucleus.

Reference:

◊  Nishimoto N.et al., J Cell Sci.125: 3870-82. pubmed/Brg1 Free article. WB, IP (2012).
 
>