Anti EDD / UBR5 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti EDD / UBR5 Antibody

Catalog No.: BAM-70-500EX
Size: 20UG
Price: [DISCONTINUED] Sorry!! This product is not available anymore.
antigen/source: EDD / UBR5
host: Rabbit
Label: Unlabeled
Purity: Affinity Purified
Application: Western Blot
Storage: -20C
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Immunogen: Human
Reacts with: Human
Mouse
Rat
Xenopus laevis

Other:

 Background 
EDD / UBR5 (2,799 aa, 309 kDa) is E3 ubiquitin-protein ligase which is a component of the N-end rule
pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to
the N-end rule, leading to their ubiquitination and subsequent degradation By similarity. Involved in maturation and/or
transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle
progression. May havetumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the
DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also
acts as a regulator of DNA damage response byacting as a suppressor of RNF168, an E3 ubiquitin-protein ligase
that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a
guard against excessive spreading of ubiquitinated chromatin at damagedchromosomes.
 Application 
1) Western blotting (1/1,000 dilution) (Fig. 1)
Not tested for other applications.
[Immunogen]
Synthetic peptide of human EDD protein corresponding to 394-408 amino acids,
C-KWSESEPYRNAQNPS, conjugated with KLH
 Cross reactivity 
Human (HeLa, HEK293T, MCF7) and mouse (NIH3T3). Note that expression level of EDD varies
greatly, depending on tissues and cell types (see Fig.1).
Not tested in other species.



 IMAGE1



Fig.1 Identification of EDD in whole cell extracts of human cells by western blotting using anti-EDD antibody. Lane 1; HeLa cells transfected with control siRNA Lane 2; HeLa cells transfected with EDD-specific siRNA Lane 3; HEK293T cells transfected with pFlag-CMV-5b empty vector Lane 4; HEK293T cells transfected with pCMV-Tag2b-EDD expression vector Predicted molecular mass of EDD is 309 kDa and the corresponding band in Lane 1 is much reduced in Lane 2 by introduction of the EDD-specific siRNA. Note that expression level of EDD in HEK293T cells is much higher than in HeLa cells. The data are by courtesy of Prof. M. Fujita of Kyushu University

 IMAGE2



Fig.2. Immunofluorescence staining of EDD protein in MCF7 cells. MCF7 cells were fixed in 4% paraformaldehyde overnight and permeabilized in 0.25% TritonX 100 in PBS for 10 min. Anti-EDD antibody was used at 1/1,000 dilution. As second antibody, goat anti-rabbit IgG conjugated with Alex488 was used at 1/1,000 dilution. DNA was stained with DAPI.@Note that not all nuclei were stained, indicating cell cycle dependency of EDD protein existence.
 
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