Collagen Glycation Assay Kit, Glucose / Fructose - Cosmo Bio Co.,Ltd.

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Collagen Glycation Assay Kit, Glucose / Fructose

Catalog No.: PMC-AK70-COS
Size: 1KIT
Price: ¥36000
$480
Storage: 4C
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Purpose: For a long term examination that researches early saccharification from fructose.
component: Collagen Acidic Solution, 5 mL
Neutralizing Solution, 5 mL
Glucose Solution (200 mM), 10 mL
Fructose Solution (200 mM), 10 mL Sample Dilution Buffer 20 mL
Aminoguanidine Solution (20 mM): Positive Control, 0.1 mL
* One kit contains regents for 192 assays (96 well Plate)
* Additional materials required
- 96well black plateiclear bottomAsterilej
Greiner [μCLEAR|PLATE BLACK Cat.No.655090] is recommended.
- Fluorescent microplate reader
(Mode: Fluorescence Bottom Reading, Excitation Wavelength: 370 nm, Emission Wavelength: 440 nmj

Other:

 Background 
The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids, and nucleic acids) is called the Maillard reaction or glycation. This reaction is initiated by the nonenzymatic reaction of reducing sugars with free amino groups on proteins to form Amadori products. The Amadori products undergo a variety of irreversible dehydration and rearrangement reactions, leading to the formation of advancedglycation end products (AGEs). AGEs have adverse effects on the functional properties of proteins. Many AGEs have fluorescent and covalent cross-linking properties. The accumulation of AGEs is thought to play an important role in the pathogenesis ofdiabetic patients and the aging process.
The collagen that forms bone, skin, and blood vessel is also glycated.
 Principle 
The collagen gel formed in 96-well plates generates fluorescence after long-term incubation with glucose or fructose at 37‹C. Some reagents or natural products inhibit this reaction. The Collagen Glycation Assay Kit, Glucose / Fructose is a complete assay system, which is designed to measure fluorescent AGEs using a fluorescence micro plate reader equipped with a 370 nm excitation filter and a 440 nm emission filter.


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Reference:

•  Masayoshi Takeuchi et al., Mol Med. 2000 Feb;6(2):114-25. PMID: 10859028
•  Jun-ichi Takino et al., J Gastroenterol. 2010 Jun;45(6):646-55. PMID: 20084527
 
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