Fecal Mucin Assay Kit - Cosmo Bio Co.,Ltd.

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Fecal Mucin Assay Kit

Catalog No.: CSR-FFA-MU-K01E
Size: 1KIT
[100 TEST]
Price: ¥42000
$560
Storage: 4C
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Purpose: This kit extracts mucin in feces and is refined and cuts O-glycosidic linkage under an alkali condition with provided mucin, and a carbohydrate chain reducing group can measure the mucin content that is in feces by measuring intensity of fluorescence to be provided by reacting 2-cyano acetamide at the same time.
component: Buffer solution A (tablet): *3 for 100 mL
Buffer solution B (acetic acid): *1 25 mL
Buffer solution C (boric acid): *1 25 mL
Reagent A (2-cyano acetamide): *1 1.0 mL
Reagent B (NaOH):) *2 1.5 mL
Standard solution (N-acetylgalactosamine, 250 ug / mL ): *1 1.0 mL
Enzyme solution: *1 1.5mL

Other:

 Background 
Mucins are a family of heavily glycosylated proteins, and main components of mucosa such as saliva, tear, gastric fluid enteric fluid. Basic configuration of mucin are a macromolecules linked ramiform sugar chain to peptide framework.The heterogeneous property of sugar chain makes them diversity, the molecules has various function, such as specific molecular recognition.
Some of the sugar chains recognize a specific protein derived from virus, bacteria was found. Mucins are positioned in mucosal barrier function in gut, stopping the translocation of pathogen and toxin into blood vessel beyond the intestinal wall. This kit is useful for determination of mucin content in feces.
Mucins are a family of high molecular (1000kda-10000kda) and heavily glycosylated protein. Mucin domains within the protein core are rich in threonine, serine and hydroxyproline, reducing end of sugar chain (GalNAc) are frequentry-linked to these amino acid by the post-translational O-glycosylation. This kit contains components to determine fecal mucin content.
Step1: Extraction and partial purification of mucin from feces.
Step2: Determination of mucin O-glycosidically linked oligosaccharide chains is ƒŔ-eliminated by diluted alkali, and reducing end of sugar chain is formed. Reducing carbohydrates react at high temperature with 2- cyanoacetamide (CAN) to produce intensity fluorescent condensate.
This product is designed for the measurement in fluorescence plate leaders (96 well plates).
When using Fluorescence plate leader, it can measure 100 samples.
When you measure with a light spectrophotometer, please use it with a microcell.




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Reference:

•  Susumu Honda, Yoshikazu Matsuda, Masaye Takahashi, and Kazuaki KakehiFluorimetric Determination of Reducing Carbohydrates with2-Cyanoacetamide and Application to Automated Analysis of Carbohydrates as Borate Complexes. (1980) Analytical Chemistry,Vol.52, No.7
•  Bovee-Oudenhoven IM, Termont DS, Heidt PJ, et al.: Increasing the intestinal resistance of rats to the invasive pathogen Salmonella enteritidis: additive effects of dietary lactulose and calcium. Gut 40: 497-504, 1997.
•  CrowtherRS, Wetmore RF: Fluorometric assay of O-linked glycoproteins by reaction with 2-cyanoacetamide. Anal Biochem 163: 170-174, 1987.
•  Okazaki Y, Han Y, Kayahara M, Watanabe T, Arishige H, Kato N. Consumption of curcumin elevates fecal immunoglobulin A,an index of intestinal immune function, in rats fed a high-fat diet. J Nutr Sci Vitaminol (2010);56(1):68-71.
•  Yukako Okazaki,Hiroyuki Tomotake, Kazuhisa Tsujimoto, Masahiro Sasaki,and Norihisa Kato. Consumption of a Resistant Protein, Sericin, ElevatesFecal Immunoglobulin A, Mucins, and Cecal Organic Acids in Rats Fed a High-Fat Diet. (2011) The Journal of Nutrition, 21, 10.3945/jn.111.144246.
•  Zaki Utama & Yukako Okazaki & Hiroyuki Tomotake & Norihisa Kato, Tempe Consumption Modulates Fecal Secondary Bile Acids, Mucins, Immunoglobulin A, Enzyme Activities, and Cecal Microflora and Organic Acids in Rats. Foods Hum Nutr (2013) 68:177-183
 
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