Anti Keratan Sulfate (R-10G) - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Keratan Sulfate (R-10G) Antibody

Catalog No.: CAC-RIT-M001
Size: 100UG
Price: ¥40000
$534
antigen/source: iPS cell (Tic)
host: Mouse
Purity: Ig fraction - Protein G
Application: Western Blot
Immuno Fluorescence
Immunocytochemistry (cell)
Storage: -70C
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Immunogen: Human
Isotype: IgG1
Clone: R-10G
Reacts with: Human
Purpose: R-10G is useful as a potent tool for the evaluation and standardization of hiPS cells in regenerative medicine.

Other:

 Background 
Monoclonal antibody R-10G recognises Keratan Sulfate lacking oversulfated structures in oligosaccharide segments of
Keratan Sulfate glycosaminoglycan chains on hiPS cells.Digestion with Keratanase II, Keratanase or Endo-B-galactosidase
removes these epitopes from Keratan Sulfate proteoglycans.
R-10G is useful as a potent tool for the evaluation and standardization of hiPS cells in regenerative medicine
 Application 
E Western blotting: 3 g / mL
E Immunohistochemistry : 10 g / mL
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.
 Cross reactivity 
Human, Other species have not been tested.
 Specificity 
R-10G epitopes are oligosaccharide segments of Keratan Sulfate glycosaminoglycan chains
consisting of disaccharide-repeating units with galactose and 6-sulfated N-acetyl-glucosamine,
lacking oversulfated structures. Digestion with Keratanase II, Keratanase or
Endo-B-galactosidase removes these epitopes from Keratan Sulfate glycosaminoglycan chains.
R-10G epitopes are expressed on human iPS/ES cells but not on human EC cells.



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Localization of the R-10G(Green) and TRA-1-81(Red) epitopes on cultured Tic cells

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Fig.1 Screening of R-10G mAb by western blotting. Tic cell lysates in the complete RIPA buffer were desolved by SDS-PAGE on a 4-15% gradient gel under nonreducing, followed by immunoblot detection with R-10G. A : GelCode* Blue staining of SDS-PAGE of the Tic cell lysates (10ug protein). B : Tic cell lysates were analyzed by western blotting with R-10G. The molecular mass markers are shown on the left.

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Fig. 2. Localization of the R-10G and TRA-1-81 epitopes on cultured Tic cells visualized on laser confocal microscopy.Tic cells cultured on Millicell* EZ slides were double-stained first with R-10G and Alexa Fluor 488-conjugated secondary (anti-mouse IgG1) antibody, followed by with TRA-1-81 and Alexa Fluor 555-conjugated secondary (anti-mouse IgM) antibody. Cells were observed at two different magnifications: ~100 (upper panel) and ~400 (lower panel). (I) Nomarski imaging. (II) Nuclear counterstaining with TO-PRO*-3. (III) Antigens for R-10G (green). (IV) Antigens for TRA-1-81 (conventional hiPS marker antibody) (red). (V) Merged image of (III) and (IV). (VI) Close-up view of V (~400).

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Pluripotent cell epitope on the polycadixin molecular structure

Reference:

•  Kawabe K.et al.,.Glycobiology 23 (3), 322-336 (2013), PMID: 23154990
•  Schopperle WM, et al, Stem Cells. Mar;25(3):723-30 (2007). PMID: 17124010
 
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