Anti SUMO 2/3 - Cosmo Bio Co.,Ltd.

Antibodies

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Anti SUMO 2/3 Antibody

Catalog No.: BAM-70-657-EX
Size: 100UG
Price: ¥29000
$387
antigen/source: SUMO 2/3
host: Rat Mono
Application: Western Blot
Immunocytochemistry (cell)
IHC frozen section
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Immunogen: Human
Isotype: IgG2a
Reacts with: Human
Simian
Mouse
Hamster
Rat



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Fig.1. Detection of SUMO-2/3 by Western blotting with anti-SUMO2/3 antibody (3H12). High molecular multiple bands were observed in HeLa total cell extract. As secondary antibody, Alkaline phosphatase conjugated anti-rat IgG was used.

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Fig.2. Detection of SUMO-2/3 in whole cell extracts of mammalian cells by Western blotting with anti-SUMO2/3 antibody (3H12). 1. MCF-7 (human breast cancer cell line) 2. NIH3T3 (mouse fibroblast cell line) 3. CHO (Chinese Hamster Ovary cell) 10-20% gradient gel was used for SDS-PAGE. Wet blotting method was employed. Anti-SUMO-2/3 antibody (3H12) was used at 1/1,000 dilution. As a second antibody, goat anti-rat IgG antibody conjugated with HRP was used at 5,000 dilution. *Arrow indicates unconjugated SUMO-2/3 proteins. SUMO-2/3 proteins conjugate numerous proteins in vivo, and SUMOylation states vary depending on the kinds of cells and physiological states of them.

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Fig.3 Immunofluorescence staining of SUMO-2/3 with the anti-SUMO2/3 antibody (3H12) in the mouse primary neural progenitor cells. DNA was stained with Hoechst.

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Fig.4 SUMO-2/3 foci detection in C-33A cells by imunofluorescence staining with anti-SUMO-2/3 antibody (3H12). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.25 TritonX-100. As secondary antibody, Alexa 488 conjugated donkey anti-rat IgG was used. Cells were analyzed using Olympus IX71 microscope and Lumina Vision software (Mitani Co., Tokyo)

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Fig.5 Immunohistochemistry of Coronal section of E16.mouse cerebral cortex. Coronal section was immunostained with anti-SUMO-2/3 antibody (3H12). DNA was stained with Hoechst.
 
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