Anti Calreticulin-3/CALR3/Calsperin - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Calreticulin-3/CALR3/Calsperin Antibody

Catalog No.: BAM-73-022-EX
Size: 100UL
Price: ¥34500
$460
antigen/source: Calreticulin-3/CALR3/Calsperin
host: Rabbit
Application: Western Blot
Immunoprecipitation
Immunohistochemistry
Storage: -20C
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Reacts with: Mouse



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Fig.1. Testeis specific expression of Calreticulon-3 as examined in various tissues by western blotting with anti-CALR3 antibody. The various tissues were excised and homogenized in lysis buffer containing 1% TritonX100 and then placed on ice for 1 h. These extracts were centrifuged, and the supernatants were collected and analyzed by western blotting with anti-CALR3 antibody at 1/1,000 dilution. 1. Brain. 2. Lung. 3. Heart. 4. Thymus. 5. Liver. 6. Spleen. 7. Kidney. 8. Muscle. 9. Ovary. 10. Testis. 11. Sperm

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Fig.2. Identification of Calreticulon-3 protein by western blotting with anti-CALR3 antibody. Embryonic fibroblast cells prepared from Calr3 -/- mouse were transfected with a plasmid expressing Calr3. The cell lysate was analyzed by western blotting with anti-CALR3 antibody at 1/1,000 dilution. 1. Mock-infected cell lysate as a negative control. 2. Cell lysate transfected with a plasmid expressing Calr3.

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Fig.3 Immunoprecipitation of Calreticulon-3 protein with anti-CALR3 antibody. Lysates of wild-type mouse testis were immunoprecipitated with anti-CALR3 antibody and the precipitates were analyzed by western blotting with the same antibody. 1. Input testis lysate 2. Precipitated with preimmune serum 3. Precipitated with anti-CALR3 antibody

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Fig.4 Immunofluorescence staining of a testicular section with anti-CALR3 antibody.Sequential sections were stained with hematoxylin and eosin (upper panel). CALR3 was detected in elongating spermatids (lower panel). Testis was collected from adult mouse and fixed in 4% paraformaldehyde/PBS overnight at 4 C, cryopreserved in graded 10?30% sucrose, and embedded. Frozen sections (8 µm) were mounted on aminopropyl triethoxysilane-coated glass slides. Primary antibody was used at 1/100 and as secondary antibody, Alexa Fluor 488 conjugated goat anti-rabbit IgG was used. Scale bar is 200 µm.
 
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