Anti PDILT - Cosmo Bio Co.,Ltd.

Antibodies

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Anti PDILT Antibody

Catalog No.: BAM-73-051-EX
Size: 100UL
Price: ¥34500
$460
antigen/source: PDILT
host: Rabbit
Application: Western Blot
Immunoprecipitation
IHC frozen section
Storage: -20C
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Immunogen: Mouse
Reacts with: Mouse



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Fig. 1 Analysis of PDILT protein in testes extracts from wild-type and Pdilt knock-out mice by western blotting with anti-PDILT antibody. Sperm was lyzed in lysis buffer containing 1% Triton-X100 and extracts were prepared as supernatants of lysates after centrifugation. Samples were reacted with anti-PDILT antibody at 1/1,000 dilution. -Tubulin was used as a control.

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Fig.2 Western blotting analysis of PDILT expression in various tissues with anti-PDILT antibody.Proteins were extracted from various tissues with lysis buffer containing Triton X-100 and subjected to western blot analysis. Tissue proteins (30 ug) and sperm protein (10 ug) were reacted with anti-PDILT antibody at 1/1,000 dilution.

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Fig.3 Immunoprecipitation of PDILT protein from mouse testis extracts. Extracts (100 µg) of wild-type (+/+) and Pdilt (-/-) mouse testes were immunoprecipitated with anti-PDILT antibody at 1/100 dilution and the precipitates were analyzed by western blotting using the same antibody at 1/1,000 dilution. 1. Input. 2. Wild-type. 3. Pdilt (-/-)

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Fig.4 Immunofluorescence staining of mouse testicular sections with anti-PDILT antibody. Testes were collected from adult mice and were fixed in 4% paraformaldehyde/PBS, cryopreserved in garaded 10-30% sucrose, and embedded in a TissueTek OCT compound (Sakura Finetechnical, Tokyo). Frozen sections (5 µm) were mounted on APS-coated glass slides. After washing and blocking, the slides were reacted with anti-PDILT antibody at 1/100 dilution. As a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG antibody was used. PDILT was detected only in spermatid .
 
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