GIP (Total) ELISA - Cosmo Bio Co.,Ltd.




Catalog No.: YII-YK254-EX
Size: 1KIT
Price: ¥79000
antigen/source: GIP
Application: Enzyme Linked Immunosorbent Assay
Storage: 4C
Immunogen: Rat
Measurement Range: 3.1-200 pM


The incretin hormones, glucose-dependent insulinotropic polypeptide (GIP) and glucagons-like
peptide-1 (GLP-1), are a group of gastrointestinal hormones that cause an increase in the amount of
insulin released from the beta cells of the islets of Langerhans after ingestion of food.
The intestinal peptide GIP was first isolated from porcine upper small intestine1). The sequences of
porcine2) 3), bovine4) and human GIP5) have been determined, each has 42 amino acids, andthe sequences
is highly conserved. The porcine and bovine peptides differ from the human at two and three site,
respectively. Takeda et al. have isolated a human cDNA encoding the GIP precursor and confirming that
GIP belongs to the vasoactiveintestinal peptide (VIP)/Glucagon/secretin family6). GIP is a
gastrointestinal peptide hormone that is released from duodenal endocrine K cells after absorption of
glucose or fat7). GIP is a potent releaser of insulin in experimental animals8) andin man 9,10) provided
that the blood glucose is above basal level. Plasma level of GIP is elevated after an oral glucose load or a
meal in normal man. This increase after a meal is below normal in newly diagnosed insulin?dependent
diabetics11).It is now being recognized that GIP receptor is also expressed in organs and cells such as
duodenum, small intestine, pancreatic alpha-cell, adipocyte and osteoblast. These results demonstrate
GIP may have a lot of physiological effect in additionto their glucoregulatory effects12,13,14,15).
GIP is rapidly inactivated by the enzyme dipeptidyl peptidase- 4 (DPP- 4) to GIP (3-42) with a blood
half-life of only several minutes. DPP- 4 inhibitor can prolong the half-life of GIP, that expecting
treatment of incretin effect.
The kit can be used for measurement of total GIP [both GIP (1-42) and GIP (3-42)] in rat plasma with
high sensitivity. It will be a specifically useful tool for incretin research.
ThisELISAkit for determination of rat total GIP is based on a sandwich enzyme immunoassay. To the
wells of plate coated with highly purified mouse monoclonal antibody against rat GIP, standards or
samples are added for the 1st step immunoreaction. Afterthe1st step incubation and plate washing, HRP
labeled antibody solution against rat GIP is added as the 2nd step to form antibody - antigen - labeled
antibody complex on the surface of the wells. After the 2nd step incubation and rinsing out excess
labeled antibody, Finally, HRP enzyme activity is determined by 3,3f,5,5f-Tetramethylbenzidine (TMB)
and the concentration of rat total GIP is calculated.
This ELISA kit is used for quantitative determination of rat totalGIPin plasma and culture medium supernatant. The kit is characterized by its sensitive quantification and high specificity. In addition, it has no influence by other components in samples. GIP standard is highly purified synthetic product.


Performance Characteristics


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