L-Glutamine Assay Kit - Cosmo Bio Co.,Ltd.

Antibodies

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L-Glutamine Assay Kit

Catalog No.: YMS-80116
Size: 1KIT
Price: ¥160000
$2134
Storage: 4C
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Purpose: To measure L-glutamine removing effect of L-glutaminic acid
component: R1 enzyme reagent liquid: 1 vial, 30mL
R2 enzyme reagent liquid: 1 vial, 30mL
L-glutamine standard solution: 1 vial (lyophilized)

Other:

 Features 
%This product is a measurement kit of L-glutamine using L-glutaminic acid oxidation enzyme 1). Even if L-glutamine coexists with L-glutaminic acid in a sample, it can specifically assay only L-glutamine. In addition, it can assay L-glutamine by the operation that is simpler than a method with L-glutaminic acid dehydrogenase. All the necessary reagents are set, and a cage, R1 enzyme reagent liquid and the R2 enzyme reagent liquid are in condition to be usable immediately.
 Principle 
When adding R1 enzyme reagent liquid in a sample, L-glutaminic acid is oxidized by the action of the L-glutaminic acid oxidation enzyme and produces hydrogen peroxide. Because hydrogen peroxide is disintegrated by the action of the catalase, L-glutaminic acid included in in a sample is removed. L-glutamine in a sample is broken down into L-glutaminic acid by action of the glutaminase. Next, when adding R2 enzyme reagent liquid , L-glutaminic acid is oxidized by the action of the L-glutaminic acid oxidation enzyme, and produces hydrogen peroxide. As for produced hydrogen peroxide, a purple pigment is generated by a reaction of a color development substrate and peroxidase. From this purple absorbance (555nm), it assay L-glutaminedensity in a sample. In addition, the catalase deactivates it by sodium azide just after R2 enzyme reagent liquid addition promptly.



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L-glutamine calibration curve It can measure L-glutamine in the range of 10-1500 mg/L.

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Result of L-glutamine solution measurement adding L-glutaminic acid

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Result of L-glutamine solution measurement adding L-glutaminic acid

Reference:

Arima J et al., J Biochem 134, 805-812, 2003
 
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