Fig.1 Flow cytometry: Untransfected (red) and transfected with ICAFectinR
441 and plasmid encoding mouse
CD163 Hela cells were stained with rabbit anti-mouse CD163 polyclonal antibodies diluted 1/100 (orange)
and 1/200 (blue) and R-PE goat anti rabbit IgG secondary antibody at the dilution 1/200.
Fig.2 Immunohistochemistry: Hela cells transfected with
441 and plasmid encoding mouse CD163 were fixed 24h post transfection, permeabilized and
stained with rabbit anti-mouse CD163 Polyclonal Antibodies diluted 1/20 and Alexa488 goat anti rabbit IgG
secondary antibody at dilution 1/1000. Nuclei were counterstained with DAPI in blue.