Anti PRDX - Cosmo Bio Co.,Ltd.

Antibodies

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Anti PRDX Antibody

Catalog No.: CAC-ICA-TG6-MSP1
Size: 50UL
Price: ¥60000
$800
antigen/source: PRDX
host: Mouse
Label: Unlabeled
Purity: Serum
Application: Enzyme Linked Immunosorbent Assay
Immunohistochemistry
Flow Cytometry
Storage: -20C
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Immunogen: Mouse
Isotype: IgG
Reacts with: Mouse
component: Formulation Buffer /Form Liquid: (Preservative : None)
Volume: 50 L

Other:

 Background 
Peroxiredoxin-4 is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the
cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water
and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has
been found to play a regulatory role in the activation of the transcription factor NF-kappaB.
[Immunogen]
ICANtibodiesTM encoding mousePRDX4 full length nucleic acid sequence
 Specificity 
mouse PRDX4
 Application 
Immunohistochemistry: 1/20
Flow cytomerty: 1/100
ELISA: 1/500
Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.



 IMAGE1



Fig.1 Immunohistochemistry: Hela cells transfected with ICAFectin441R and plasmid encoding mouse PRDX4 were fixed 24h post transfection, permeabilized and stained with mouse anti-mouse PRDX4 Polyclonal Antibodies diluted 1/20 and Alexa488 goat anti mouse IgG secondary antibody at dilution 1/1000. Nuclei were counterstained with DAPI in blue.

 IMAGE2



Fig.2 Flow cytometry: Untransfected (black) and transfected with ICAFectinR 441 and plasmid encoding mouse PRDX4 (red) Hela cells were fixed 24 hours post transfection, permeabilized and stained with mouse anti-mouse PRDX4 Polyclonal Antibodies diluted 1/100 and R-PE goat anti mouse IgG secondary antibody at the diltion 1/200.

 IMAGE3



Fig.3 ELISA: Lysates of untransfected and transfected HeLa cells with ICAFectinR 441 and plasmid encoding mouse PRDX4 were coated on MaxiSorp plates (Nunc) in bicarbonate buffer. Determination of anti-mouse PRDX4 Polyclonal IgG in the mouse sera was performed by using HRP goat anti mouse IgG secondary antibody at the dlition 1/5000.
 
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