S1 Nuclease - Cosmo Bio Co.,Ltd.

Antibodies

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S1 Nuclease

Catalog No.: OZK-OZ-40-EX
Size: 200UL
Price: ¥10000
$134
Storage: -20C
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 Background 
S1 Nuclease is a single-stranded-specific endonuclease that hydrolyzes single-stranded
DNA and RNA into acid-soluble 5f- mononucleotides. It hydrolyzes single-stranded regions in duplex DNA such as loops and gaps.
This S1 Nuclease is from Aspergillus sp.
[SPECIFICATIONS]
Storage.....................................10mM sodium acetate, pH4.6
150mM NaCl
0.05M ZnSO4
50% glycerol
Activity....................................100 units/l
Molecular Weight........................32,000
glycoprotein containing Zn2+
Optimum pH and temperature..... See Fig. 1, 2
pH and thermal stability ............ See Fig. 1, 2
[ASSAY]
Unit Definition
One unit of enzyme hydrolyzes 1g of heat-denatured calf thymus DNAinto acid-soluble
form at 37C, pH4.6 in one minute.
Composition of Supplied Reagents (Store at -20C):
10~S1 Nuclease Buffer............300mM Sodium acetate, pH4.6
2800mM NaCl
10mM ZnSO4



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Reference:

1)Lee, B. R., Kitamoto, K., Yamada, O. and Kumagai, C. (1995) Appl Microbial Biotechinol, 44, 425-431. 2)Vogt, V.M. (1973) Eur. J. Biochem, 33, 192-200. 3)Berk, A. J. and Sharp, P. A. (1978) Proc. Natl. Acad. Sci. USA, 75, 1274-1278. 4)Wiegand, R.C., Godson, G. N. and Radding, C. M. (1975) J. Biol. Chem., 250, 8848-8855.
 
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