Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric) - Cosmo Bio Co.,Ltd.

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Glucose Cellular Uptake Measurement Kit (Broad Range, Fluorometric)

Catalog No.: CSR-MBR-PMG-K01E
Size: 100TEST
Price: ¥105600
$1408
Storage: -20C
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Purpose: A complete reagent set to measure 2DG in cells after cell lysis by sonication
component: Reagents
2DG6P Solution (1 mM)500 L3 x 3 ml
-20C
(protect from light)
Sample Diluent Buffer Concentrate (100x)5 ml1 vial
Substrate Buffer9 mL1 vial
Fluorescent Substrate120 L1 vial
Enzyme Solution270 L1 vial
Materials required but not supplied.
Ultrapure water (distilled water)
2-deoxyglucose suggested product
Bovine Serum Albumin suggested product
96-well black wall microplate
96-well fluorescent plate reader
Suitable sonication device for cell lysis.

Other:

 Background 
Insulin is a hormone that lowers the blood glucose level and is known to help glucose enter cells through an effect that enhances the activity of glucose transport, primarily in insulin-sensitive cells, such as adipocytes and skeletalmuscles.
The effect of insulin and other growth factors on cells is often assessed in the laboratory by measuring glucose uptake activity. Typically, glucose uptake is measured using 3-O-Methyl-D-Glucose or 2-deoxyglucose (2DG) labeled with radioactive 3H or other isotopes. However, the use of radioactive isotopes is not available to all labs and is subject to many restrictions. This assay kit was developed to provide a simple, rapid and convenient means to measure cellular glucose uptakewithout the use of radioisotope.



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Reference:

>Yamamoto N, et al ,(2006). A nonradioisotope, enzymatic assay for 2-deoxyglucose uptake in L6 skeletal muscle cells cultured in a 96-well microplate. Anal. Biochem. 351: 139-145.
 
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