RAGE Reactive AGEs Assay Kit, Glyceraldehyde - Cosmo Bio Co.,Ltd.

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RAGE Reactive AGEs Assay Kit, Glyceraldehyde

Catalog No.: CSR-AAS-AGE-K04E
Size: 1KIT
Price: ¥150000
$2000
Storage: 4C DNF
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Purpose: This kit is designed to detect GA-AGE formed on BSA using recombinant Fc-RAGE as detection reagent.
This kit will be useful not only for biomarker assessment and drug screening for drug development but also assessing theactivity of functional foods and other ingested substances.
component: 1. 96-well albumin coated plate 1 Plate (One strip-well plate)
2. Microplate sealing fi lm 96 well, 2 sheets
3. Sample Dilution Buff er 30 mL
4. Glyceraldehyde solutioni100mMj 5 mL
5. Aminoguanidine Stock Solution (20mM)
¦ Positive control 0.5 mL
6. Washing Buff eri10X) 30 mL
7. Blocking Buff er 10 mL
8. RAGE-Fc Solution 5 mL
9. Alkaline Phosphatase (ALP) labeled protein A/G 5 mL
10. Substrate Tablets (For 5mL) 3 tablets
11. Substrate Dilution Buff er 15 mL

Other:

 Background 
Although carbohydrates are indispensable for ATP production, excess carbohydrates promote protein glycation. Protein
glycation may result in irreversible changes to protein structure, net charge, and protein function with deleterious
consequences. Protein glycation has been associated with diabetic complications, renal disorders, Alzheimer?s disease and
others.
First described Louis Camille Maillard in 1912, glycation is also referred to as the Maillard reaction. Maillard reactions are
categorized into early and advanced stages. Early stage reactions generate Amadori rearrangement products such as
haemoglobin A1c, whereas advanced stage reactions generate the so-called AGEs (advanced glycation end products)
characterized by protein-crosslinking and color browning. Collagens, important structural proteins of skin, blood vessel walls
and bone, are subject to glycation.
Indeed, different types of receptors recognize AGEs as ligands. CD36, a scavengerreceptor expressed on macrophages
and vascular endothelial cells binds AGEs (mainly the CML form) and ox-LDL for clearing metabolic products resulting from
oxidative stress. SR-A is also recognizes AGEs, Ox-LDL and Ac-LDL and this binding suggesteda strong relationship between
AGEs and arteriosclerotic diseases and diabetic nephropathy.
The so-called RAGE receptor (Receptor for AGEs) was found
and isolated in 1992 from bovine lung as 55kDa membranespanning
protein. (1) RAGE expression isdistributed. RAGE is
present not only on monocytes and or macrophages but also
on vascular smooth muscle cells, neurons, hepatocytes, and
kidney mesangial cells.
The function of AGE receptors are still unclear but have been
suggested to effect signal transductions or the apoptosis
process. RAGE itself does not bind binds to AGE types and
exhibits high specificity to glycelaldehyde-derived AGEs (GAAGE).
GA-AGE has been suggested to be a "toxic AGEh (TAGE).
Such TAGEs may be particularly detrimental to cells and may be
more highly associated with disease or disease complications.
In addition, recent reports describe glycosaminoglycan (GAG)
sulfates on some cancer cells as RAGE ligands and suggest that
such interactions maypromote tumor metastasis (2,3).
Thus, factors effecting GA-AGE formation, and compounds
effecting the interaction of GA-AGE with RAGE are of interest to study links between AGEs and disease and for the
development of drugs that inhibit AGEs-relatedpathophysiologies.



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Preparation of Reagents

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Protocol

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Example of Results

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Reference:

•  Schmidt, A. M. et al., J. Biol. Chem. 267, 14987-14997, 1992.
•  S. Mizumoto et al., J. Biol. Chem. 287 (23), 18985-18994, 2012.
•  A. Nishikawa, T. Taira, K. Yoshizato. (1987) Exp. Cell Res. 171, p164-177.
•  H. Shoda et al., (1997) Endocrinology 138, p1886-1892.
•  Jun-ichi Takino et al., (2010) J. Gastroenterol 45: 646-655.
•  M. Takeuchi (2012) Folia Pharmacol. Jpn. 139, p193-197.
•  S. Mizumoto et al., FEBS Journal., Vol. 280 Issue 10, p2462-2470, 2013.
 
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