Anti Tetanus Toxin - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Tetanus Toxin Antibody

Catalog No.: BAM-64-035-EX
Size: 100UL
Price: ¥34500
$460
antigen/source: Tetanus Toxin
host: Mouse
Application: Western Blot
Enzyme Linked Immunosorbent Assay
Storage: -20C
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Isotype: IgG1
Clone: TH-11

Other:

 Background 
Tetanus toxin acts by inhibiting neurotransmitter release. It binds to peripheral neuronal synapses, is internalized and moves by retrograde transport up the
axon into the spinal cord where it can move between postsynaptic and presynaptic neurons. It inhibits neurotransmitter release by acting as a zinc endopeptidase that catalyzes the hydrolysis of the '76-Gln-|-Phe-77' bond of synaptobrevin-2.
 Application 
1. Western blotting (1/1,000 dilution)
2. ELISA (assaydependent)
3. Neutralising of the toxin activity (assay dependent)
Other applications have not been tested.



 IMAGE1



Figure. Western Blot analysis of Tetanus Toxin with anti-Tetanus Toxin antibody (TH11) Left Panel is SDS-PAGE analysis of partially purified tetanus toxin, silver stained, and Right panel is western blot analysis of the same samples. Lane 1 sample was reduced and Lane 2 sample was unreduced. Tetanus toxin Heavy and Light chains are so tightly attached and they are difficult to separate completely by heating in SDS in the presence of mercaptoethanol. In the above reduced samples, part of the reduced sample remained attached as shown by gH+Lh in lane 1 of Left panel . To dissociate the two attached fragments completely, addition of 2M urea is required The antibody (TH11) recognizes Light chain of the toxin. The anti-tetanus toxin antibody was used at 1/1,000 dilution.
 
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