Anti Legionella pneumophila - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Legionella pneumophila Antibody

Catalog No.: BAM-64-100-EX
Size: 200UL
Price: ¥34500
$460
antigen/source: Legionella pneumophila
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Immunogen: Legionella pneumophila

Other:

 Background 
Legionnaires disease (LD) was recognized in 1976 after an outbreak of pneumonia at an American Legion convention in Philadelphia. Soon after, the etiologic agent was identified as a fastidious gram-negative bacillus and named Legionella pneumophila. Although several other species of the genus Legionella were subsequently identified, L pneumophila is the most frequent cause of human legionellosis and a relatively common cause of community-acquired and
nosocomial pneumonia in adults. In children, L pneumophila is also an important, although relatively uncommon, cause of pneumonia.
 Application 
1) Immunofluorescent and Immunochemical staining (1/10,000 ~1/30,000 dilution)
2) Immunohistochemistry (1/3,000~1/10,000)
3) ELISA (1/10,000~1/30,000 dilution)
4) Agglutination (1/2,000~1/5,000)



 IMAGE1



Fig.1 Immunofluorescent staining of Legionella pneumophila in the infected HEK293 cells using anti-Legionella pneumophila antibody. HEK293 cells were infected with Legionella pneumophila strain Philadelphia1 fixed with 4% formaldehyde and reacted with the anti-Legionella pneumophila antibody at 1/10,000 dilution. As the second antibody, goat Rodamine Red X conjugated anti-rabbit IgG antibody was used at 1/10,000 dilution. DNA was stained with Hoechst 33342 (center) and the images were merged with that of differential interference contrast microscope (right).

 IMAGE2



Fig.2. Titration of antibody reactivity of anti-Legionella pneumophila antiserum by direct ELISA Plate was coated with 100 l of 109 cells/ml per well and 100 l of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate. gpreh is preimmune serum and gImmunizedh is immunized serum.
 
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