Anti Influenza B Virus HA - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Influenza B Virus HA Antibody

Catalog No.: BAM-65-160-EX
Size: 100UG
Price: ¥34500
$460
antigen/source: Influenza B Virus HA
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Immunogen: Influenza Virus
Clone: 1H12

Other:

 Background 
Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. ClassIviral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating
the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformationalchange in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a
competent fusion pore.
 Application 
1) Western blotting (1/500~1/1,000 dilution)
2) Immunofluorescent and Immunocytochemical staining (1/100~1/200 dilution)
3) Immunoprecipitation (1/200 dilution)
4) ELISA (assay dependent)
 Specificity 
According to Ref.1 during epidemic in Osaka 1996-97, 1H12 antibody reacted with HA protein of most of Influenza B virus isolates belonging to Victoria group tested (70/73 strains) and none of clinical 27 isolates belonging to Yamagata group as examined by PAP staining. However later test with IF staining it also reacts with some of Yamagata group strains like B/Florida/4/2006. However, note that HA changes during passages and may change reactivity to this antibody.
No cross reactivity with any strains of influenza A viruse.



 IMAGE1



Fig. 1 Detection of HA protein in the crude extracts of MDCK cells infected with various Influenza B virus strains by western blotting using 1H12 monoclonal antibody. 1. B/Mie/1/1993 2. B/Johannes Burg/5/1999 3. B/Florida/4/2006 4. B/Lee/1940 5. B/Florida/4/2006 6. B/Lee/1940 7. B/Malasia/2506/2004 8. B/Massachusetts/2/2012 First antibody was used at 1/500 dilution and as 2nd antibody, HRP-conjugated goat anti-mouse IgG antibody was used at 1/10,000 dilution. Positions of marker proteins are indicated in kDa on the left. Clone 1H12 recognizes an epitope on HA2 region.

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Fig.2 Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 1H12). Anti-Influenza B Virus HA antibody (clone 1H12) efficiently detected the viruses in the infected MDCK cells with B/Malaysia/2506/2004 and B/Florida/4/2006 virus strains. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody (green). Image on the left is a negative control, mock-infected MDCK cells.
 
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