Anti Influenza B Virus HA - Cosmo Bio Co.,Ltd.

Antibodies

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Anti Influenza B Virus HA Antibody

Catalog No.: BAM-65-165-EX
Size: 100UG
Price: ¥34500
$460
antigen/source: Influenza B Virus HA
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Immunogen: Influenza Virus
Clone: 10B8

Other:

 Background 
Hemagglutinin (HA) binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.
 Application 
1) Western blotting (1/500~1/1,000 dilution)
2) Immunofluorescent and Immunocytochemical staining (1/100~1/200 dilution)
3) Immunoprecipitation (1/200 dilution)
4) Neutralization of infectivity (NT) (assay dependent)
5) Hemagglutination Inhibition (HI) (assay dependent)
6) ELISA (assay dependent)
 Specificity 
According to Ref.1 during epidemic in Osaka 1996-97, 10B8 antibody reacted with HA protein of all Influenza B virus isolates belonging to Victoria group tested (73 strains) and none of clinical 27 isolates belonging to Yamagata group as examined by PAP staining. Italso reacts with Victoria group vaccine strains; Shangdong/7/1997, Malasia/2506/2004. However, note that HA changes during passages and may change reactivity to this antibody. By western blotting, reactivity with B/Malasia/2506/2004 and B/Massachusetts/2/2012 was tested positive.
No cross reactivity with any strains of influenza A viruse.



 IMAGE1



Figure Immunofluorescence assay of MDCK (canine kidney ) cells infected with Influenza B virus, using anti-Influenza B virus HA antibody (clone 10B8). Samples were taken at 24 hours post-infection. Anti-Influenza B Virus HA antibody (clone 10B8)) efficiently detected HA in the B/Malasia/2506/2004 virus (Victorial group) but not in B/Florida/4/2006 virus (Yamagata group) infected MDCK cells. The cells were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) and permeabilized with 0.1% Triton X-100 in PBS. The bound antibody was visualized by a further reaction with an Alexa Fluor 488-conjugated secondary antibody. Images on the left are mock-infected MDCK cells as negative control.
 
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