Atelocollagen, Honeycomb sponge

The 'Honeycomb' collagen sponge has a structure in which uniform pores (200-400 μm) are arranged densely in one direction, into which cells can penetrate and proliferate. This structure facilitates the ready supply of nutrients to the cells inside the sponge, and releases metabolic wastes and biochemical products. Cells can proliferate and fill the lumen to form a uniform cell mass.

CSH-10 : stereoscopic microscope image

CSH-96 : stereoscopic microscope image

Characteristics of Atelocollagen FAQ
Atelocollagen is a collagen solubilized by protease, but its physical properties are virtually identical to those of natural, unsolubilized collagen. Furthermore, atelocollagen additionally has superior characteristics;----- More

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The 'Honeycomb' collagen sponge is prepared from highly purified bovine dermal type I Atelocollagen and can be degraded by collagenase.

• Three dimensional culture
• Research for tissue engineering as 3D scaffold

Atelocell instruction manual for Atelocollagen, Honeycomb sponge

Electron microscope image of Honeycomb sponge

Electron microscope image of mouse fibroblast cell culture in 'Honeycomb collagen sponge

<References>
1. Biodegradable honeycomb collagen scaffold for dermal tissue engineering George J, Onodera J, Miyata T, J Biomed Mater Res A. 2008 Dec 15;87(4):1103-11.
2. Bone tissue engineering using human adipose-derived stem cells and honeycomb collagen scaffold. Kakudo N, Shimotsuma A, Miyake S, et al. J Biomed Mater Res A. 2008 Jan;84(1):191-7
3. Growth inhibition and differentiation of cultured smooth muscle cells depend on cellular crossbridges across the tubular lumen of type I collagen matrix honeycombs. Suzuki T, Ishii I, Kotani A, et al.　Microvasc Res. 2009 Mar;77(2):143-9
4. Rodriguez AP, MissanItoh H, Aso Y, Furusea L, Nagatsuka H, et al.:Efficacy of atelocollagen honeycomb scaffold in bone formation using KUSA/A1 cells. J Biomed Mater Res A, 77(4):707-17. 2006
5. George J, Kuboki Y, Miyata T, et al.:Differentiation of mesenchymal stem cells into osteoblasts on honeycomb collagen scaffolds. Biotechnol Bioeng, 95(3):404-11.2006
6. Imamura T, Cui L, Teng R, et al.:Embryonic stem cell-derived embryoid bodies in three-dimensional culture system form hepatocyte-like cells in vitro and in vivo. Tissue Eng, 10(11-12):1716-24. 2004
7. Ishii I, Tomizawa A, Kawachi H, et al.:Histological and functional analysis of vascular smooth muscle cells in a novel culture system with honeycomb-like structure. Atherosclerosis, 158(2):377-84. 2001
8. Itoh H, Aso Y, Furuse M, et al.:A honeycomb collagen carrier for cell culture as a tissue engineering scaffold. Artif Organs, 25(3):213-7. 2001
9. Moriyama T, Asahina I, Ishii M, et al.:Development of composite cultured oral mucosa utilizing collagen sponge matrix and contracted collagen gel: a preliminary study for clinical applications. Tissue Eng, 7(4):415-27. 2001