useful for study of interacting between cells

Atelocollagen, Permeable membrane for 6-well, 24-well culture plate

Background

Permeable collagen membrane is specially developed from highly purified bovine dermal type I Atelocollagen for single and double layer tissue culture studies. It is particularly suitable for studying the molecular interactions between two different cell types by culturing on both sides of the membrane. It is potentially useful for the study of artificial organs and in the emerging field of tissue engineering. The membrane is permeable and allows free passage of amino acids and small molecules, which is important for the absorption and exchange of molecules through the membrane using cell polarity.
This Collagen membrane is available for use with both 6 and 24 well culture plates.

AteloCell™ Flyer PDF

Feature and Advantages

• Due to membrane transparency, microscopic observation of the cells is possible while culturing.
• Study of cell interaction is possible without a co-culture.
• Cell culturing on a collagen membrane creates a natural environment that resembles in-vivo systems.
• Collagen membranes is available for use with both 6 and 24 well culture plates.
• Amino acids and other small molecular weight compounds can move freely though the permeable collagen membrane.
• All products are manufactured under stringent quality control.Collagen membranes
• All product are sterilized.

Application

• Study of interaction between cells
• Fundamental researches of the artificial internal organs
• Screening of efficacy of medicine
• Study of cell metabolism and metabolism mechanism

Instruction manual for Atelocollagen, Permeable membrane for 50mm culture dish PDF PDF

<References>
1) Oyasu M, Fujimiya M, Kashiwagi K, et al Immunogold electron microscopic demonstration of distinct submembranous localization of the activated gammaPKC depending on the stimulation. J Histochem Cytochem.2008 Mar;56(3):253-65
2) Furuta A, Miyoshi S, Itabashi Y, et al. Pulsatile Cardiac Tissue Grafts Using a Novel Three Dimensional Cell Sheet Manipulation Technique Functionally Integrates With the Host Heart, InVivo Circ Res. 2006 Mar 17;98(5):705-12.
3) Orisaka M, Mizutani T, Tajima K, et al.:Effects of ovarian theca cells on granulosa cell differentiation during gonadotropin-independent follicular growth in cattle. Mol Reprod Dev, 73(6):737-44. 2006

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Products List (Relative Products)

	Product Name	Cat#	Quantity	Price
	Atelocollagen membrane for 6-well culture plate ,	KOU-CM-6	24 PC	¥42,000 $443 €400
	Atelocollagen membrane for 24-well culture plate ,	KOU-CM-24	24 PC	¥18,400 $194 €176

KOU-CM6
Collagen membrane for tissue culturing for 6-well culture plates, sterile

KOU-CM24
Collagen membrane for tissue culturing for 24-well culture plates, sterile

Atelocollagen，Permeable membrane <100mm×90mm×35μm>

Collagen membrane for research is a highly purified, bovine dermal type I atelocollagen applicable to various purposes.
* Since the product will be manufactured upon order receipt, please inquire about the delivery date.

pH 3.0,
100mm × 90mm × 35um, sterile

	Product Name	Cat#	Quantity	Price
	Atelocollagen membrane ,	KOU-CLF-01	1 SHEET	¥32,400 $342 €309

< Kit for delivering siRNA, Nucleic acid, Antibody and Protein >

AteloGene™
This product is a kit for delivering siRNA into animal tissues by local or systemic administration in mice and then efficiently introducing it into the cells
[KOU-1392, KOU-1393]
Genom ONE-NEO
GenomONE™-Neo_EX is a tool for transfection of molecules (plasmid DNAs, siRNAs,　oligonucleotides, proteins, antibodies etc.) into cells and animal tissue by means of membrane fusion.
[ISK-GN-001-EX, ISK-GN-004-EX]
Genom ONE-CAb
GenomONE™-CAb_EX is a tool for efficient introduction of IgG antibodies into living cells using HVJ-E envelope (HVJ-E) vector for the purpose of analysis of the functions of cells and intracellular proteins.
[ISK-AB001EX, ISK-AB004EX]

< Collagen for Cell Culture : AteloCell™ >

Atelocollagen, Native collagen, Atelocollagen powder
Collagen solution for tissue culturing is a highly purified collagen solution manufactured in KOKEN(Tokyo) advanced laboratory with excellent quality control.
[KOU-IPC-30, KOU-IPC-50, IAC-30, IAC-50, KOU-CLP-01]
Atelocollagen, Eagle's MEM,Hanks' Medium,DMEM, Atelocollagen powder
Atelocollagen, Eagle's MEM,Hanks' Medium, and DMEM are highly purified collagen solution with medium.
[KOU-MEN-02, KOU-HAN-02, KOU-DME-02, KOU- CLP-01]
Collagen microspheres
Collagen microspheres are a cell culture substrate prepared from bovine dermal type I Atelocollagen. This product can be used for the culturing , such as fibroblasts, epithelial cells and osteoblasts, and has proven effective for the long-term maintenance of cell cultures.
[KOU-MIC-00]
Atelocollagen, Honeycomb sponge
The 'Honeycomb' collagen sponge is prepared from highly purified bovine dermal type I Atelocollagen and can be degraded by collagenase.
[KOU-CSH-10]
Collagen sponge for 35mm culture dish, Atelocollagen sponge
The collagen sponge is a collagen-based device developed for three-dimensional cell culturing.
[KOU-CS-35, KOU-CLP-01]
Atelocollagen sponge, MIGHTY <coming soon>
MIGHTY is a collagen sponge with strength that doesn't collapse even if the compressive load of 30kPa (single times) is added.
[KOU-CSM-25, KOU-CSM-50]
Atelocollagen, Permeable membrane for 50mm culture dish, Atelocollagen membrane
Collagen membrane is specially developed from highly purified bovine dermal type I Atelocollagen for single and double layer tissue culture studies.
[KOU-MEM-01, KOU-CLF-01]
Atelocollagen, Permeable membrane for 6-well，24-well culture plate, Atelocollagen membrane
Due to membrane transparency, microscopic observation of the cells is possible while culturing.
[KOU-CM-6, KOU-CM-24, KOU-CLF-01]
Type II Collagen
Usefull for tissue and cell culture
[KOU-CL22]
Atelocollagen powder, Atelocollagen sponge, Atelocollagen membrane <Order to be made>
These products are useful for researcher's various research purposes.
[KOU-CLP-01, KOU-CLS-01, KOU-CLF-01]