Next-generation RACE

accura-expRACE KIT


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1st-strand cDNA synthesis → 2nd-strand cDNA synthesis → RACE PCR

From cDNA Synthesis to RACE in only a Day.

 

Results of RACE experiment 
5’ RACE of the mTfrc gene by using mouse testis ds cDNA synthesized with this kit. 
M: λ Hind III digestion marker
Lane 1: full-length cDNA ~4.4 kb 
Lanes2, 3: ~3 kb cDNA

 


accura-expRACE KIT flyer [PDF]

Background

◊In RACE (Rapid Amplification of cDNA Ends) by the single-primer method* of this kit, the targeted cDNA is amplified by PCR with only a gene-specific primer using ds cDNA as a template.
◊The mechanism is based on that the terminal region of the ds cDNA is partially denatured at 68 C for the extension reaction and that the linear DNA molecule tends to circularize.
◊Upon reaching the 5’ end of the template DNA, thermostable DNA polymerase switches templates to the 5' terminal region of the newly synthesized daughter strand at a certain probability and synthesizes DNA sequences complementary to the gene-specific primer.
◊Using this daughter strand as a template, the targeted cDNA is amplified with only a gene- specific primer.

*US patent #7504240
Japanese patent #4304350

Feature and Advantages

♦By using the accura-expRACE KIT, both 5’ and 3’ RACE can easily and efficiently be performed under simple conditions, such as RT-PCR.
♦ Long cDNA and rare cDNA unidentified previously can be isolated.
♦ The synthesized ds cDNA can be used as a cDNA library. You can perform ~400 screenings of a cDNA library by RACE.
♦ The cDNA synthesized by this kit contains a high proportion of full-length cDNA because an “advanced type M-MLV Reverse Transcriptase” is used.

Performance comparison with another brand kit

Performance comparison result (1) by a third party entity

Performance comparison result (2) by a third party entity

Product List

Product Name Cat# Quantity Price

accura-expRACE KIT DataSheet ,

ELP-EPI001-EX

5 RXN see also
# ELP-EPI6201EX
Size 5

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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