Rapid detection of fluorescent AGEs and inhibition assay for glycation

Albumin Glycation Assay Kit


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Background

The non-enzymatic reaction of reducing carbohydrates with lysine side chains and N-terminal amino groups of macromolecules (proteins, phospholipids and nucleic acids) is called the Maillard reaction or glycation. The products of this process, termed advanced glycation end products (AGEs), adversely affect the functional properties of proteins. Many AGEs have fluorescent and convalent cross-linking properties.


Accumulation of AGEs has been thought to play an important role in the pathogenesis of diabetic patients as well as the aging process. 

Recent studies have suggested that AGEs can arise not only from sugars but also from carbonyl compounds derived from the autoxidation of sugars and other metabolic pathways. Among different AGEs, there is evidence that glyceraldehyde-derived AGEs are associated with such cytotoxcity.

Albumin Glycation Assay Kit provides rapid detection of fluorescent AGEs and inhibition assay for glycation of albumin solution by glyceraldehyde.This kit provides sufficient reagents to perform up to 192 assays.

The availability and convenience of this kit will contribute to analysis of functional foods or development of anti-glycation material in the cosmetic field.

Feature and Advantages

œThis kit provides sufficient reagents to perform up to 192 (96×2)assays.
¦This kit does not include a 96 well plate.
œQuick assay (Assay can be completed in one day)
œEasy protocol with measurement by fluorescence plate leader. (Excitation wavelength 370nm, fluorescence wavelength 440nm)
œThis kit can assay 2 plates of 96 well.
œSuitable for screening and developing raw materials that suppress the glycation process!

Kit component

Albumin Glycation Assay Kit

Kit Components Volume Number Storage
Bovine Serum Albumin (BSA) Solution 10 mL 1 bottle 4°C
glyceraldehyde (500mM) 2 mL 1 bottle
buffer solution 30 mL 1 bottle
Aminoguanidine solution (20mM)
*Anti glycation standard substance
0.5 mL 1 bottle

* One kit contains regents for 192 assays (Tube or 96 well Plate)
* Additional materials required
E 96well black plate (clear bottom, sterile)
Greiner [μCLEAR|PLATE BLACK Cat.No.655090] is recommended.
E Fluorescent microplate reader
(Mode: Fluorescence Bottom Reading, Excitation Wavelength: 370nm, Emission Wavelength: 440nmj

Experimental Results

Principle

Albumin Glycation Assay Kit is a complete assay system designed to measure the fluorescent AGEs using the fluorescence microplate reader equipped with a 370nm excitation filter and 440nm emission filter.

References

(1) Masayoshi Takeuchi et al. Immunological Evidence that Non-carboxymethyllysine Advanced Glycation
End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo. Mol Med.
2000 Feb;6(2):114-25. PMID: 10859028

(2) Jun-ichi Takino et al. The formation of intracellular glyceraldehyde-derived advanced glycation
end-products and cytotoxicity. J Gastroenterol. 2010 Jun;45(6):646-55. PMID: 20084527

Product List

Product Name Cat# Quantity Price

Albumin Glycation Assay Kit, Glyceraldehyde DataSheet ,

CSR-AAS-AGE-K01E

1 KIT ¥45,000
$600

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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