Anti-Calreticulin / CALR


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Background

Calreticulin (CALR) is calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis.

Application

Identification of CALR protein by western blotting with anti-CALR antibody.
Embryonic fibroblast cells prepared from Calr -/- mouse were transfected with a plasmid expressing Calr. The cell lysate was analyzed by western blotting with anti-CALR antibody at 1/500 dilution. 1. Mock-infected cell lysate as a negative control. 2. Cell lysate transfected with a plasmid expressing Calr.

 

 

Immunoprecipitation of CALR protein with anti-CALR antibody.
Lysates of wild-type mouse testis were immunoprecipitated with anti-CALR antibody and the precipitates were analyzed by western blotting with the same antibody.
1. Input testis lysate
2. Precipitated with preimmune serum
3. Precipitated with anti-CALR antibody

References

> Ikawa M., et al. Calsperin is a testis-specific chaperone required for sperm fertility. J Biol Chem. 2011 Feb 18;286(7):5639-46. PMID: 21131354

Product List

Product Name Cat# Quantity Price

Anti Calreticulin/CALR (Rabbit)  DataSheet

BAM-73-018-EX

100 UL ¥34,500
$460

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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