Anti-Nup62


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Background

The nuclear pore complex (NPC) regulates cargo transport between the cytoplasm and the nucleus. Nucleoporins are the main components of the NPC in eukaryotic cells. Nup (Nucleoporin) 62 (522 aa, 53 kDa) is a member of the FG-repeat containing nucleoporins and is localized to the NPC central plug. Nup62 associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Predicted to contain about 10 N-acetylglucosamine side chain.

Application

 

Fig.1 Detection of Nup62 in membrane fraction of HeLa cells by Western blotting with the antibody 2A. Sample is the nuclear membrane fraction of HeLa cells.?@The antibody was used at 1/500 dilution. As a second antibody, alkaline phosphatase conjugated anti-rat IgG antibody was used The protein migrates at 60 kDa position.

 

Fig.2 Immunofluorescent staining of HeLa cells with the antibody 2A, focused on nuclear surface. HeLa cells were fixed with 3.7% formaldehyde and permeabilized with 0.5% Triton X-100. The anti-Nup62 antibody (2A) was used at 1/400 and as a second antibody, Alexa 488 conjugated goat anti-rat IgG antibody was used at 1/500 dilution.

 

Fig.3. Immunofluorescent staining of HeLa cells with the antibody 2A, focused on nuclear rim. Methods are as described in Fig.2.

References

>Fukuhara T et al gFunctional analysis of nuclear pore complex protein Nup62/p62 using monoclonal antibodies.h Hybridoma 25: 51-59 (2006) PMID 16704304
> Maeshima K et al gCell-cycle-dependent dynamics of nuclear pores: pore-free islands and lamins.h J Cell Sci 119: 4442-4451 (2006) PMID 17074834

Product List

Product Name Cat# Quantity Price

Anti Nup62, Human (Rat Mono) Unlabeled DataSheet

BAM-70-305EX

200 UG ¥34,500
$460

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.