For@dementia and tauopathy research

Anti 4R-tau Antibody


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Background

Tau is a microtubule-associated protein. In adult human brain, 6 tau isoforms are expressed, which are divided into two groups, 3-repeat (3R) and 4-repeat (4R) tau isoforms. Tau assembles into insoluble, filamentous, and hyperphosphorylated inclusions in many neurodegenerative diseases, and different tau isoforms are accumulated in the diseased brains, namely, 6 tau isoforms in AD, 3R tau isoforms in Pick’s disease, and 4R tau isoforms in PSP and CBD. Therfore, isoform-specific tau antibodies are useful tools for immunohistochemical and biochemical studies of tau species in diseased brains. Tau deposited in AD is extensively deamidated at N279. This anti-4R specifically recognizes 4R tau isoforms regardless of deamidation and strongly stained tau in AD brain.

Application

EWestern blotting: 1/2000 - 1/5000

EImmunohistochemistry: 1:3000 - 1/20000

Antibody Characterization

Fig1: Immuno blot analysis for 6 kinds of tau isoforms (3-repeat and 4-repeat) with anti total tau (T46) and  Anti 4R (TIP-4RT-P01).

Fig2: Immuno staining for paraffin embedded section of cerebral cortex prepared from AD brain (retrieved with proteinase K and formic acid)

References

1. Spillantini MG, Goedert M. Lancet Neurol. 2013 Jun; 12(6):609-22.
2. Umeda Y, et al., Neurosci Lett. 2004 Apr 15; 359(3): 151-4.
3. Dan A, et al., Acta Neuropathol Comm 2013; 1: 54.
4. Hasegawa M, et al., Acta Neuropathologica 2013; Nov 9.

Product List

Product Name Cat# Quantity Price

Anti 4R-tau, Human (Rabbit) Unlabeled DataSheet

CAC-TIP-4RT-P01

50 UL ¥50,000
$667

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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