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Anti ACE3 [Angiotensin converting enzyme-like 3 ]


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Background

ACE3 was identified as an IZUMO1 interacting protein from mouse sperm. ACE3 protein is exclusively localized in testis and sperm. However, unlike the disruption of IZUMO1 or ACE, elimination of ACE3 in mouse did not result in loss of sperm fertilizing ability.

Application

 

Fig.1. Expression of ACE3 in Testis and sperm of mouse as identified by western blotting with anti-ACE3 antibody. All samples were loaded at 30 µ‚‡ protein and detected with anti-ACE3 antibody at 1/500 dilution.

 

Fig.2. Immunoprecipitation of ACE3 protein from sperm lysate. ACE3 protein was immunoprecipitated from total sperm protein (~100 µg) with anti-ACE3 antibody and detected by western blotting with anti-ACE3 antibody.
1. Non-immune serum.
2. Anti-ACE3 antibody

 

 

Fig.3. Subcellular localization of ACE3 protein in sperm. Mouse sperm were prepared from cauda epidymis, fixed with ethanol and stained with anti-ACE3 antibody at 1/100. As a secondary antibody, Alexa Fluor 546 conjugated anti-rabbit IgG.

References

# Inoue N. et al., Identification and disruption of sperm-specific angiotensin converting
enzyme-3 (ACE3) in mouse. PLoS One. 2010 Apr 22;5(4):e10301. PMID: 20421979

Product List

Product Name Cat# Quantity Price

Anti ACE3, Mouse (Rabbit)  DataSheet

BAM-73-006-EX

100 UL ¥34,500
$460

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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