Anti CD52


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Background

CD52 may play a role in carrying and orienting carbohydrate, as well as having a more specific role. Expressed on lymphohematopoietic tissues, including thymus, spleen, and bone marrow, but not in liver, kidney, and brain.

Applications

Fig.1. Western blotting analysis of CD52 expression in various tissues with anti-CD52 antibody. Testes, male reproductive ducts and sperm protein were extracted with lysis buffer containing Triton X-100 and subjected to western blot analysis. Western blots containing equal amounts of tissue proteins (30 ug) and sperm protein (10 ug) were reacted with anti-CD52 antibody at 1/1,000 dilution.

 

Fig.2. Western blot analysis of CD52 in cauda epididymal lysates and sperm lysates of wild type and CD52 deficient mice. Cd52+/+ (+/+), Cd52+/- (+/-), and Cd52-/-(-/-).Cauda epididymis and sperm from vas deferens were lysed in lysis buffer containing 1% TritonX-100. Proteins (30 µg for cauda epididimis and 10 µg for sperm) were analyzed by western blotting with anti-CD52 antibody at 1/1,000 dilution.

References

# Yamaguchi R., et al. Cd52, known as a major maturation-associated sperm membrane antigen secreted from the epididymis, is not required for fertilization in the mouse. Genes Cells. 2008 Aug;13(8):851-61. PMID: 18782223

Product List

Product Name Cat# Quantity Price

Anti CD52, Mouse (Rabbit)  DataSheet

BAM-73-030-EX

100 UL ¥34,500
$460

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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