Anti NG2 / CSPG4 (2161D7) Antibody


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NG2, also known as HMW-MAA or MCSP and encoded by the CSPG4 gene, is a unique transmembrane proteoglycan that may be accounted for many of the interactions taking place between cells and their microenvironment during both cell propagation and cell movement. In the adult human body, NG2 has a relatively limited tissue distribution and is characteristically found with prevalence on immature and progenitor cells of various tissues and organs, including brain, skeletal muscle, cartilage and skin. It is generally absent from all mature epithelial and hematopoietic cells, while it becomes de novo expressed upon neoplastic transformation of different cell type with the most striking examples being its appearance on melanocytes converting into melanoma and T lymphocytes and myeloid cells turning into leukemic cells.

Application

WB, IP, IHC(P), ELISA
Western blotting, 1/10 to 1/30 (Distinct band at 250-260 kD)
Immunoprecipitation, 1/5 - 1/10
Immunohistochemistry, 1/5 to 1/50 (paraffin-embedded) *
ELISA, 1/10 - 1/150
*
The antibody stains preferentially vascular structures and progenitor cells of skin, cartilage and muscle, oligodendrocyte precursors, certain epithelial cells (precursors); and pericytes and certain tumour cells in melanoma, breast carcinoma and soft-tissue sarcoma lesions. Other applications have not been tested. Optimal dilutions/concentrations should be determined by the end user.

 

( Immunostainings of a malignant glioblastoma A) and cutaneous melanoma C) lesion. B) Western blotting on whole tissue/cell lysates of human glioblastoma lesion and the human melanoma A375 cell line after SDS-PAGE under reducing conditions on 4-10% gradient gels. D) immunostaining of human melanoma MeWo cells. Nuclear counterstasting was performed with Hoescht.

 

Product List

Product Name Cat# Quantity Price

Anti NG2 / CSPG4 (2161D7), Human (Mouse) Unlabeled DataSheet

CAC-PRPG-NG-M01

2 ML ¥50,000
$667

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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