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Anti 1-Oleolyl-Parmitoyl-Phosphatydylcholine (OPPC) Antibody


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Background

The main constituents of biological membrane are the glycerophospholipids. Besides variety in their hydrophilic portions, phospholipids comprise various molecular species with different fatty acid moieties. Among those varieties,this antibody selectively binds to the phosphatidylcholine with oleic acid at the sn-1 site. This type of lipid is concentrated at the tip of neuronal protrusions.

Application

◊ Immno-electron microscopy: 3 ug/ml ( Ref.1, Fig. 1 )

◊ Fluorescence - activated cell sorter: 1 ug/ml ( Ref. 1, Fig. 3 )

◊ Immuno-fluorescence cell staining: 0.3 ug/ml ( Ref.1, Fig. 1 )

◊ ELISA: 3 ug/ml ( Ref.1, Fig. 2 )

Other applications have not been tested.
Optimal dilutions/concentrations should be determined by the end user.

Fig.1 OPPC localization analysis on cultured nerve cell(rat PC12 cell, clone#15-3C1)

Description

Immunogen : PC12 raft fraction

Rased in : Mouse (Balb/c)

Myeloma : PAI

Clone number : 15-3C1

Isotype @@@@@@: IgM

Source : Culture supernatant (+10%FBS)

Buffer : RPMI+0.025% NaN3 as a preservative

Concentration  : 90 ug / ml

Volume@@@@    : 0.5 ml

Label@              : Unlabeled

Specificity        : 1-Oleoyl-phosphatidylcholine

Antigen@          : PC12 raft fraction

Storage          @: 4°C

References

1) Kuge H. et al. Functional Compartmentalization of the Plasma Membrane of
Neurons by a Unique Acyl Chain Composition of Phospholipids J. Biol. Chem. 289
(2014) PMID:25096572

Product List

Product Name Cat# Quantity Price

Anti 1-Oleoyl-Palmitoyl-Phosphatidylcholine (OPPC) (Mouse) Unlabeled DataSheet

CAC-SK-KC01-M01

0.5 ML see also
# CAC-SKKC01M01
Size 200

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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