Antibodies for Hsc70/Hsp70


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Background

Heat shock cognate protein 70 (HSC70) is a constitutively expressed housekeeping protein and molecular chaperone which belongs to the heat shock protein 70 (HSP70) family. HSC70 interacts with many other substances and also regulates many cellular functions. It is also involved in various diseases and may become a biomarker for diagnosis and potential therapeutic targets for design, discovery, and development of novel drugs to treat various diseases1

1 Liu T et al, Pharmacol Ther. 2012 Dec;136(3):354-74

Applications

 

Antibody to heat shock cognate protein (HSC70, 651 aa; gene, At5g02500)
Antiserum dilution: 1/500.
Protein sample: radish (Raphanus sativa L.Tokinashi-daikon), crude membrane fraction


5 µg of total protein from (1) cow muscle, (2) chicken muscle, (3) pig muscle, (4) rat liver, (5) salmon muscle , (6) seal muscle, (7) mummichog heat shock control, (8) mummichog heat shock post-24 hours, extracted with Protein Extration Buffer, PEB (AS08 300), were separated on 4-12% NuPage (Invitrogen) LDS-PAGE and blotted 1h to PVDF. Blots were blocked immediately following transfer in 2% ECL Advance blocking reagent (GE Healthcare) in 20 mM Tris, 137 mM sodium chloride pH 7.6 with 0.1% (v/v) Tween-20 (TBS-T) for 1h at room temperature with agitation. Blots were incubated in the primary antibody at a dilution of 1: 10 000 for 1h at room temperature with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed once for 15 min and 3 times for 5 min in TBS-T at room temperature with agitation. Blots were incubated in secondary antibody (Agrisera anti-rabbit IgG horse radish peroxidase conjugated, AS09 602) diluted to 1:50 000 in 2% ECL Advance blocking solution for 1h at room temperature with agitation. The blots were washed as above and developed for 5 min with ECL Advance detection reagent according to the manufacturers instructions. Images of the blots were obtained using a CCD imager (FluorSMax, Bio-Rad) and Quantity One software (Bio-Rad). Exposure time was 30 seconds.

Product List

Product Name Cat# Quantity Price

Anti HSC70 (Rabbit)  DataSheet

COP-COP-080016

100 UL ¥30,000
$400

Anti Hsc70, - (Rabbit)  DataSheet

AGR-AS05 062

100 UL ¥112,000
$1494

Anti HSC70/HSP70, Wheat (Rabbit)  DataSheet

AGR-AS07 233

100 UL ¥112,000
$1494

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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