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  • in vivo siRNA transfection kit "AteloGene® Local Use Quick Gelation" - Kit component

Useful for delivering siRNA into animal tissues by local

in vivo siRNA transfection kit "AteloGene® Local Use Quick Gelation" - Kit component


Kit component

Contents of kit
This kit is intended for 15 times of administration.

1) Prefilled syringe (filled with “AteloGene® QG
Each syringe is for 5 times of administration, including losses during the preparation steps.
540 µL × 3 syringes
2) QG buffer: 2.5 mL × 1 bottle
3) 2 mL microtube:
A spare tube is included. 
4 tubes
4) 18G needle (for ejection and suction):
2 spare needles are included.
8 needles
5) Instruction manual: 1 leaflet


Devices and reagents required other than the kit

- 1 mL dispossable syringe
- 27G needle (for injection)
- siRNA/miRNA (PAGE- or HPLC- purified grade)
- Container for preparation of siRNA/miRNA solution (sterilized, RNase free)
- Cooling device (crushed ice, cold block, etc.)
- Tube rotator (that can tumble and agitate such as TAITEC RT-5, Bibby scientific SB3, etc.)
- Pipetter and tips (sterilized, RNase free)
- High-speed refrigerated centrifuge
- Anesthetic


Storage temperature: 2-10°C(do not freeze)
Precautions for storage


- Gelation and thermal denature occur in "AteloGene®" at a temperature higher than 20°C. Do not use "AteloGene®" that was once gelated or thermally denatured.
- Freezing “AteloGene®” causes bubbles in the mixture and the dispersion of components. Never use “AteloGene®” that was once frozen.


Precautions for use


Do not use “AteloGene® ” for any purpose other than research use. Application to the human body is strictly prohibited.


Be sure to read the instruction manual before use. The manufacturer is not liable for the results of usage by methods other than that described in the instruction manual. The expected effects may not always be obtained depending on the siRNA/miRNA sequences, administration targets or methods.

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.

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