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in vivo siRNA transfection kit "AteloGene® Local Use Quick Gelation" - Methods


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"AteloGene Local Use Quick Gelation" Preparation Instruction 

In this section, the number 1)-4) refer to the "Kit contents" of the product page. Implement measures to secure an RNase-free environment, as for as possible, to avoid the degradation of siRNA/miRNA in advance.

1)Preparation of "AteloGene® Local Use Quick Gelation"

Attach the 4)18G needle to the 1)Prefilled syringe. Eject the whole amount (540 µL) into the 3)2 mL microtube. After ejection, cool the microtube containing ”AteloGene® QG” on ice.

Note) An excess amount of “AteloGene® QG” is provided in the 1)Prefilled syringe so that 540 µL of “AteloGene® QG” can be injected into the 3)2 mL microtube regardless of losses during the preparation steps.

2)Preparation of siRNA solution

Prepare 25-50 µM siRNA/miRNA solutions with RNase-free water.

3)Preparation of "AteloGene®" & siRNA mixed solution.

While cooling on ice, gently pour 540 µL of the 2)QG buffer and then 120 µL of the siRNA/miRNA solution onto 540 µL of “AteloGene® QG” in the 3)2mL microtube. Rotate and mix the solutions at 4°C for 10 minutes. To avoid forming bubbles, the rotation speed should be approximately 12 r.p.m. (in the case of a rotator with a diameter of 20 cm).

Note) Although the mixture may become cloudy during this procedure, it will become a clear solution as the components are thoroughly mixed by rotation.

4) Deformation of bubbles and preparation for administration

Centrifuge the mixture at 10,000 r.p.m. for 1 minute at 4Ž to deform bubbles. Attach the 4)18G needle to the disposable syringe and slowly draw the mixture while avoiding forming bubbles. Replace the needle with the 27G needle and keep the syringe refrigerated until immediatelly before administration.

Mechanism of siRNA transfection using " AteloGene®."  Mechanism

Product List

Product Name Cat# Quantity Price

AteloGene(R) Local Use Quick Gelation DataSheet ,

KOU-1492

1 KIT ¥81,300
$1084

To be used for research only. DO NOT use for human gene therapy or clinical diagnosis.


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