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Anti Hepatitis Virus (HCV) core protein Monoclonal antibody


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Background

Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A, non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9,4 kb which encodes a single polyprotein of about 3,010 amino acids (1, 2, 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core, envelope1 and 2, and p7) and six non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viral replication. HCV core protein is not only a component of nucleocapsid but also has multiple functions and is thought to be a pathogenic factor for hepatitis. It also participates in some cellular processes, including transcriptional regulation and cellular transduction. HCV core antigen is used as diagnostic marker for HCV infection.

Application

1)  Western blotting
2)  Immunohistochemistry
3)  Immunofluorescence staining
4)  ELISA

Fig.1 Western blotting of HCV core protein. 
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using this antibody. The core protein is detected as a 22-kDa band.
   
Fig.2 Detection of HCV core protein by immunofluorescence antibody staining. 
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA. 
After incubation for 48 hr, the cells were fixed with acetone and HCV core protein was detected by indirect immunofluorescence staining using this antibody.
Fig.3 Immunohistochemical detection of HCV core protein. Tissue section from a patient with chronic hepatitis C was immunostained to reveal cells expressing HCV core antigen, which are scattered in the lobules (indirect immuno- histochemical method, counterstained with Mayer’s hematoxylin).
References

1. Brass V, Moradpour D, Blum HE. Molecular Virology of Hepatitis C Virus (HCV): 2006 Update. Int J Med Sci 2006; 3:29-34. PMID: 16614739
2. Kato, N. et al. (1990) gMolecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.h Proc. Natl. Acad. Sci. USA 87, 9524-9528 PMID: 2175903
3. Takamizawa, A. et al. (1991) gStructure and organization of the hepatitis C virus genome isolated from human carriers.h J.Virol.65, 1105-1113 PMID: 1847440
4. Manabe, S. et al. (1994) gProduction of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by N3.h Virology 198, 636-644 PMID: 8291245
5. Hiramatsu, N. et al. (1992) gImmunohistochemical detection of hepatitis C virus-infected hepatocytes in chronic liver disease with monoclonal antibodies to core, envelope and NS3 regions of the hepatitis C virus genome.h Hepatology, 16, 306-311 PMID: 1379209

Product List

Product Name Cat# Quantity Price

Anti HCV Core protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-051-EX

20 UG N/A

Anti HCV Core protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-052-EX

100 UG ¥34,900
$466

Anti HCV Core protein, Virus (Mouse) Biotin DataSheet

BAM-65-053-EX

50 UG ¥31,500
$420

Anti HCV Core protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-054-EX

50 UG ¥31,500
$420

Product Name Cat# Quantity Price

Anti HCV NS4a protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-056-EX

20 UG N/A

Anti HCV NS4a protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-057-EX

100 UG ¥31,500
$420

Anti HCV NS4a protein, Virus (Mouse) Biotin DataSheet

BAM-65-058-EX

50 UG ¥31,000
$414

Anti HCV NS4a protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-059-EX

50 UG ¥31,000
$414

Anti HCV NS5a protein, Virus (Mouse)  DataSheet

BAM-65-061-EX

20 UG N/A

Anti HCV NS5a protein, Virus (Mouse)  DataSheet

BAM-65-062-EX

100 UG ¥31,500
$420

Anti HCV NS5a protein, Virus (Mouse) Biotin DataSheet

BAM-65-063-EX

50 UG ¥31,500
$420

Anti HCV NS5a protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-064-EX

50 UG ¥31,500
$420

Anti HCV NS5b protein, Virus (Mouse)  DataSheet

BAM-65-066-EX

20 UG N/A

Anti HCV NS5b protein, Virus (Mouse)  DataSheet

BAM-65-067-EX

100 UG ¥31,500
$420

Anti HCV NS5b protein, Virus (Mouse) Biotin DataSheet

BAM-65-068-EX

50 UG ¥31,500
$420

Anti HCV NS5b protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-069-EX

50 UG ¥31,000
$414

Anti HCV core (CP9), Human (Mouse) Unlabeled DataSheet

SIM-2ZCP9

0.5 ML ¥64,000
$854

Anti HCV core (CP11), Human (Mouse) Unlabeled DataSheet

SIM-2ZCP11

0.5 ML ¥64,000
$854

Anti HCV core (CP14), Human (Mouse) Unlabeled DataSheet

SIM-2ZCP14

0.5 ML ¥64,000
$854

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