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Anti Hepatitis C virus (HCV) NS5b protein Monoclonal antibody


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Background

Hepatitis C virus (HCV) is a small (55-65 nm in size), enveloped, positive sense single-stranded RNA virus in the family Flaviviridae and the principal cause of parenteral non-A, non-B hepatitis. The virus genome consists of a single open reading frame of approximately 9,400 bases which encodes a single polyprotein of about 3,010 amino acids (1, 2, 3). The polyprotein is processed by host cell and viral proteases into four structural proteins (core, envelope1 and 2, and p7) and six non-structural proteins (NS2, 3, 4a, 4b, 5a, and 5b) necessary for viral replication. NS5b forms the C-terminal protein of the HCV polyprotein and functions as the viral polymerase involved in RNA replication.

Application

1. Western blotting
2. Immunofluorescence staining
3. ELISA
Other applications have not been tested.

Western blotting of HCV NS5b protein.
Fig.1  Western blotting of HCV NS5b protein.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA and were subjected to Western blotting using this antibody. The NS5b protein is detected as a 58-kDa band

Detection of HCV NS5b protein by immunofluorescence antibody staining.
Fig.2  Detection of HCV NS5b protein by immunofluorescence antibody staining.
Chimp liver cells were infected with recombinant vaccinia virus containing a HCV genome cDNA. After incubation for 48 hr, the cells were fixed with acetone and HCV NS5b protein was detected by indirect immunofluorescence staining using this antibody.

<Reference>
1. Brass V, Moradpour D, Blum HE. Molecular Virology of Hepatitis C Virus (HCV): 2006 Update. Int J Med Sci 2006; 3:29-34. PMID: 16614739
2. Kato,N. et al. (1990) “Molecular cloning of the human hepatitis C virus genome from Japanese patients with non-A, non-B hepatitis.” Proc. Natl. Acad. Sci. USA 87, 9524-9528 PMID: 2175903
3. Takamizawa, A. et al. (1991) “Structure and organization of the hepatitis C virus genome isolated from human carriers.” J.Virol.65, 1105-1113 PMID: 1847440
4. Manabe,S. et al. (1994) “Production of nonstructural proteins of hepatitis C virus requires a putative viral protease encoded by N3.” Virology 198, 636-644 PMID: 8291245

Product List

Product Name Cat# Quantity Price

Anti HCV NS5b protein, Virus (Mouse)  DataSheet

BAM-65-066-EX

20 UG N/A

Anti HCV NS5b protein, Virus (Mouse)  DataSheet

BAM-65-067-EX

100 UG ¥31,500
$420

Anti HCV NS5b protein, Virus (Mouse) Biotin DataSheet

BAM-65-068-EX

50 UG ¥31,500
$420

Anti HCV NS5b protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-069-EX

50 UG ¥31,000
$414

Product Name Cat# Quantity Price

Anti HCV Core protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-051-EX

20 UG N/A

Anti HCV Core protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-052-EX

100 UG ¥34,900
$466

Anti HCV Core protein, Virus (Mouse) Biotin DataSheet

BAM-65-053-EX

50 UG ¥31,500
$420

Anti HCV Core protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-054-EX

50 UG ¥31,500
$420

Anti HCV NS4a protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-056-EX

20 UG N/A

Anti HCV NS4a protein, Virus (Mouse) Unlabeled DataSheet

BAM-65-057-EX

100 UG ¥31,500
$420

Anti HCV NS4a protein, Virus (Mouse) Biotin DataSheet

BAM-65-058-EX

50 UG ¥31,000
$414

Anti HCV NS4a protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-059-EX

50 UG ¥31,000
$414

Anti HCV NS5a protein, Virus (Mouse)  DataSheet

BAM-65-061-EX

20 UG N/A

Anti HCV NS5a protein, Virus (Mouse)  DataSheet

BAM-65-062-EX

100 UG ¥31,500
$420

Anti HCV NS5a protein, Virus (Mouse) Biotin DataSheet

BAM-65-063-EX

50 UG ¥31,500
$420

Anti HCV NS5a protein, Virus (Mouse) Fluorescein Isothiocyanate DataSheet

BAM-65-064-EX

50 UG ¥31,500
$420

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