Tag proteins antibody

Anti GFP Monoclonal Antibody (Clone No.1A5)


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Background

The green fluorescent protein (GFP) is composed of 238 amino acids (26.9 kDa), originally isolated from the jellyfish Aequorea victoria that fluoresces green when exposed to blue light (1). In cell and molecular biology, the GFP fused gene is frequently used as a reporter of expression and protein localization (2, 3). The antibody was produced from the hybridoma cultured in serum-free medium and purified under mild conditions by propriety chromatography processes.

Application

1) Western blotting (~1삇/ml)
2) Immuno-precipitation
3) Immunocytochemistry
4) Chromatin Immuno-Precipitation (ChIP)
5) ELISA

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Immunofluorescent staing of the COS1 cells with antibody 1A5

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GFP-expressed in COS1 cells

 

<Reference>
1. Shimomura O, Johnson F, Saiga Y (1962). "Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea". J Cell Comp Physiol 59: 223–39 PMID: 13911999
2. Chalfie M, Tu Y, Euskirchen G, Ward W, Prasher D (1994). "Green fluorescent protein as a marker for gene expression". Science 263 (5148): 802–5 PMID: 8303295 
3. Tsien R (1998). "The green fluorescent protein" (PDF). Annu Rev Biochem 67: 509–44 PMID:97594961

Product List

Product Name Cat# Quantity Price

Anti GFP, - (Rat) Unlabeled DataSheet

BAM-60-001-EX

100 UG ¥26,000
$347



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