Human Epidermal Growth Factor (EGF)


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Background

EGF is a polypeptide growth hormone which plays an important role in the regulation of growth, proliferation, and differentiation of a wide range of cells by binding to its receptor EGFR (epidermal growth factor receptor). By binding to cell surface receptors, EGF activates an extensive network of signal transduction pathways that include activation of PI3K/AKT, RAS/ERK and JAK/STAT pathways.
The full-length human EGF was expressed in E.coli and highly purified by the propriety chromatographic procedures. This product has the same amino acid sequence with the mature human EGF (6.35 kDa) and has no tag attached.

Database Link : GeneID:1950, Gene Sequence:AY548762, Amino Acid Sequence:AAS83395

Application

1. EGF is commonly used as a supplement in serum-free or reduced serum media for culture of mammalian cells.
2. Studies of the human EGF receptor, transmembrane signaling and protein phosphorylation
3. Western blotting control for anti-EGF antibodies
4. Acceleration of the healing process of epidermis damage on skin, cornea, etc.
5. Widely used in cosmetics, such as whitening, anti-crinkle, anti-aging, etc.

Specification

Activity :
The ED50 as determined by a cell proliferation assay using MTS assay kit (CellTiter 96 AQueous Assay; Promega) with NHEK cells (normal human epidermal keratinocytes) was < 0.1 ng/ml, corresponding to a specific activity of < 1 x 107 units/mg.

Purity  : >95% as determined by SDS-PAGE (CBB staining)

Form   : 0.74 mg/ml in PBS (10mM Na-phosphate, 150mM NaCl) pH7.2, 50% glycerol, filter-sterilyzed 

<References>
1. Carpenter,G., Cohen,S. (1990) “Epidermal growth factor.” J. Biol. Chem. 265:7709-7712. PMID: 2186024
2. Cohen,S., Carpenter,G. (1975) “Human epidermal growth factor: Isolation and chemical and biological properties.” Proc. Natl. Acad. Sci USA 72: 1317-1321. PMID: 1055407

Product List

Product Name Cat# Quantity Price

Epidermal Growth Factor, Human DataSheet

BAM-03-001-EX

50 UG ¥12,500
$167

Epidermal Growth Factor, Human DataSheet

BAM-03-001-5EX

5*50 UG ¥37,500
$500

Product Name Cat# Quantity Price

Epidermal Growth Factor, Human DataSheet

BAM-03-001-EX

50 UG ¥12,500
$167

Epidermal Growth Factor, Human DataSheet

BAM-03-001-5EX

5*50 UG ¥37,500
$500

Fibroblast Growth Factor 1, Human DataSheet

BAM-03-003-EX

50 UG ¥26,000
$347

Fibroblast Growth Factor 1, Human DataSheet

BAM-03-003-5EX

5*50 UG ¥78,000
$1040

Keratinocyte Growth Factor (KGF/FGF7), Human DataSheet

BAM-03-005-5EX

5*50 UG ¥94,500
$1260

“Human Epidermal Growth Factor (EGF)” also belongs to the following categories.